Potassium starvation induces autophagy in yeast

被引:12
作者
Rangarajan, Nambirajan [1 ]
Kapoor, Ishani [1 ]
Li, Shuang [1 ]
Drossopoulos, Peter [1 ]
White, Kristen K. [2 ]
Madden, Victoria J. [2 ]
Dohlman, Henrik G. [1 ]
机构
[1] Univ North Carolina Chapel Hill, Dept Pharmacol, Chapel Hill, NC 27515 USA
[2] Univ North Carolina Chapel Hill, Microscopy Serv Lab, Dept Pathol & Lab Med, Chapel Hill, NC USA
基金
美国国家卫生研究院;
关键词
autophagy; starvation; potassium; yeast; electron microscopy; RNA sequencing; phosphatidylinositide 3-kinase (PI 3-kinase); transcriptomics; VPS15; PROTEIN-KINASE; SACCHAROMYCES-CEREVISIAE; FILAMENTOUS GROWTH; TRANSCRIPTION FACTOR; INHIBITS AUTOPHAGY; GENE-EXPRESSION; MAPK PATHWAYS; CELL-DEATH; 3-KINASE; COMPLEX;
D O I
10.1074/jbc.RA120.014687
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Autophagy is a conserved process that recycles cellular contents to promote survival. Although nitrogen limitation is the canonical inducer of autophagy, recent studies have revealed several other nutrients important to this process. In this study, we used a quantitative, high-throughput assay to identify potassium starvation as a new and potent inducer of autophagy in the yeastSaccharomyces cerevisiae. We found that potassium-dependent autophagy requires the core pathway kinases Atg1, Atg5, and Vps34, and other components of the phosphatidylinositol 3-kinase complex. Transmission EM revealed abundant autophagosome formation in response to both stimuli. RNA-Seq indicated distinct transcriptional responses: nitrogen affects transport of ions such as copper, whereas potassium targets the organization of other cellular components. Thus, nitrogen and potassium share the ability to influence molecular supply and demand but do so in different ways. Both inputs promote catabolism through bulk autophagy, but result in distinct mechanisms of cellular remodeling and synthesis.
引用
收藏
页码:14189 / 14202
页数:14
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