Liquid AP-UV-MALDI Enables Stable Ion Yields of Multiply Charged Peptide and Protein Ions for Sensitive Analysis by Mass Spectrometry

被引：62

作者：

Cramer, Rainer ^{[1
,3
]}

Pirkl, Alexander ^{[2
,3
]}

Hillenkamp, Franz ^{[3
]}

Dreisewerd, Klaus ^{[2
,3
]}

机构：

[1] Univ Reading, Dept Chem, Reading RG6 6AD, Berks, England

[2] Univ Munster, Inst Hyg, D-48149 Munster, Germany

[3] Univ Munster, Inst Med Phys & Biophys, D-48149 Munster, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 08期

关键词：

AP-MALDI; liquid matrices; mass spectrometry; multiply charged peptides; ASSISTED-LASER-DESORPTION/IONIZATION; ELECTROSPRAY-IONIZATION; VACUUM MALDI; DESORPTION; TRAP;

D O I：

10.1002/anie.201208628

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

All charged up: High and prolonged yields of multiply charged peptide and protein ions can be formed in MALDI mass spectrometry using liquid UV-MALDI matrices and a heated ion-transfer tube. The key features are low laser energies of 1-10 μJ, resulting in fluences of less than 200 to 2000 J m-2 and low sample ablation, high sensitivity, and continuous ion generation over tens of thousands of laser shots. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：2364 / 2367

页数：4

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