An improved LC-S/MS method for the quantitation of adenosine concentration in mice brain microdialysates

被引：7

作者：

Carrozzo, Marina M. ^{[2
]}

Troisi, Luigino ^{[2
]}

Cannazza, Giuseppe ^{[1
]}

Cazzato, Addolorata S. ^{[1
]}

Braghiroli, Daniela ^{[1
]}

Parenti, Carlo ^{[1
]}

Guiducci, Stefania ^{[3
]}

Zoli, Michele ^{[3
]}

机构：

[1] Univ Modena & Reggio Emilia, Dipartimento Sci Farmaceut, I-41125 Modena, Italy

[2] Univ Salento, Dipartimento Sci & Tecnol Biol & Ambientali, I-73100 Lecce, Italy

[3] Univ Modena & Reggio Emilia, Dipartimento Sci Biomed, Sez Fisiol, I-41125 Modena, Italy

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2012年 / 70卷

关键词：

Adenosine; LC-MS/MS; Column-switching; Microdialysis; TRANSIENT ISCHEMIA; RAT-BRAIN; INCREASES; INOSINE; PURINES; HYPOXIA;

D O I：

10.1016/j.jpba.2012.05.014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of adenosine concentrations in mouse brain microdialysis samples was developed. High method sensitivity (LLOQ of 1.25 fmol) was achieved by on-line switching column. A C18 was employed as enrichment column and a cyano based (CN-SB)as analytical column. The method was fully validated for its sensitivity, selectivity, matrix effect and stability. It was successfully applied to measure quantitatively adenosine in brain of freely moving mice after different stimuli. (C) 2012 Published by Elsevier B.V.

引用

页码：563 / 566

页数：4

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