The effects of simulated microgravity on intervertebral disc degeneration
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作者:
Jin, Li
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Univ Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USAUniv Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USA
Jin, Li
[1
]
Feng, Gang
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Univ Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USA
North Sichuan Med Coll, Nanchong Cent Hosp, Dept Orthoped Surg, Nanchong 637000, Peoples R China
North Sichuan Med Coll, Clin Inst 2, Nanchong 637000, Peoples R ChinaUniv Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USA
Feng, Gang
[1
,2
,3
]
Reames, Davis L.
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Univ Virginia Hlth Syst, Dept Neurosurg, Charlottesville, VA 22908 USAUniv Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USA
Reames, Davis L.
[4
]
Shimer, Adam L.
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Univ Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USAUniv Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USA
Shimer, Adam L.
[1
]
Shen, Francis H.
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Univ Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USAUniv Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USA
Shen, Francis H.
[1
]
Li, Xudong
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Univ Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USAUniv Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USA
Li, Xudong
[1
]
机构:
[1] Univ Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USA
[2] North Sichuan Med Coll, Nanchong Cent Hosp, Dept Orthoped Surg, Nanchong 637000, Peoples R China
[3] North Sichuan Med Coll, Clin Inst 2, Nanchong 637000, Peoples R China
[4] Univ Virginia Hlth Syst, Dept Neurosurg, Charlottesville, VA 22908 USA
BACKGROUND CONTEXT: Astronauts experience back pain, particularly low back pain, during and after spaceflight. Recent studies have described histologic and biochemical changes in rat intervertebral discs after space travel, but there is still no in vitro model to investigate the effects of microgravity on disc metabolism. PURPOSE: To study the effects of microgravity on disc degeneration and establish an in vitro simulated microgravity study model. STUDY DESIGN: Discs were cultured in static and rotating conditions in bioreactor, and the characteristics of disc degeneration were evaluated. METHODS: The mice discs were cultured in a rotating wall vessel bioreactor where the microgravity condition was simulated. Intervertebral discs were cultured in static and microgravity condition. Histology, biochemistry, and immunohistochemical assays were performed to evaluate the characteristics of the discs in microgravity condition. RESULTS: Intervertebral discs cultured in rotating bioreactors were found to develop changes of disc degeneration manifested by reduced red Safranin-O staining within the annulus fibrosus, down-regulated glycosaminoglycan (GAG) content and GAG/hydroxyproline ratio, increased matrix metalloproteinase 3 expression, and upregulated apoptosis. CONCLUSIONS: We conclude that simulated microgravity induces the molecular changes of disc degeneration. The rotating bioreactor model will provide a foundation to investigate the effects of microgravity on disc metabolism. Published by Elsevier Inc.
机构:
Xinxiang Med Coll, Affiliated Hosp 1, Dept Orthoped, Xinxiang, Peoples R ChinaXinxiang Med Coll, Affiliated Hosp 1, Dept Orthoped, Xinxiang, Peoples R China
Yang, Libin
Sun, Xiaohui
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Xinxiang Med Coll, Affiliated Hosp 1, Dept Orthoped, Xinxiang, Peoples R ChinaXinxiang Med Coll, Affiliated Hosp 1, Dept Orthoped, Xinxiang, Peoples R China
Sun, Xiaohui
Geng, Xiaolin
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Xinxiang Med Coll, Affiliated Hosp 1, Dept Orthoped, Xinxiang, Peoples R ChinaXinxiang Med Coll, Affiliated Hosp 1, Dept Orthoped, Xinxiang, Peoples R China
机构:
Department of Algology,The Third People's Hospital of YibinDepartment of Algology,The Third People's Hospital of Yibin
Tang-Hua Liu
Yan-Qing Liu
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机构:
Department of Algology,Beijing Tiantan Hospital,Capital Medical UniversityDepartment of Algology,The Third People's Hospital of Yibin
Yan-Qing Liu
Bao-Gan Peng
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机构:
Department of Orthopedics,The Third Medical Center,General Hospital of the Chinese People’s Liberation ArmyDepartment of Algology,The Third People's Hospital of Yibin
机构:
Univ Pittsburgh, Dept Orthoped Surg, Pittsburgh, PA USAUniv Pittsburgh, Dept Orthoped Surg, Pittsburgh, PA USA
Kritschil, Rebecca
Scott, Melanie
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机构:
Univ Pittsburgh, Dept Surg, Pittsburgh, PA USA
Pittsburgh Trauma Res Ctr, Pittsburgh, PA USAUniv Pittsburgh, Dept Orthoped Surg, Pittsburgh, PA USA
Scott, Melanie
Sowa, Gwendolyn
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机构:
Univ Pittsburgh, Dept Orthoped Surg, Pittsburgh, PA USA
Univ Pittsburgh, Dept Phys Med & Rehabil, Pittsburgh, PA USAUniv Pittsburgh, Dept Orthoped Surg, Pittsburgh, PA USA
Sowa, Gwendolyn
Vo, Nam
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Univ Pittsburgh, Dept Orthoped Surg, Pittsburgh, PA USAUniv Pittsburgh, Dept Orthoped Surg, Pittsburgh, PA USA
机构:
CHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea
CHA Univ, CHA Bundang Med Ctr, Dept Biomed Sci, Sch Med, Seongnam Si 13496, South KoreaCHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea
Roh, Eun Ji
Darai, Anjani
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CHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea
CHA Univ, CHA Bundang Med Ctr, Dept Biomed Sci, Sch Med, Seongnam Si 13496, South KoreaCHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea
Darai, Anjani
Kyung, Jae Won
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CHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South KoreaCHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea
Kyung, Jae Won
Choi, Hyemin
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CHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South KoreaCHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea
Choi, Hyemin
Kwon, Su Yeon
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CHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South KoreaCHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea
Kwon, Su Yeon
Bhujel, Basanta
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CHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea
CHA Univ, CHA Bundang Med Ctr, Dept Biomed Sci, Sch Med, Seongnam Si 13496, South KoreaCHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea
Bhujel, Basanta
Kim, Kyoung Tae
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机构:
Kyungpook Natl Univ, Sch Med, Dept Neurosurg, Daegu 41944, South Korea
Kyungpook Natl Univ Hosp, Dept Neurosurg, Daegu 41944, South KoreaCHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea
Kim, Kyoung Tae
Han, Inbo
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CHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South KoreaCHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea