The effects of simulated microgravity on intervertebral disc degeneration

被引:39
|
作者
Jin, Li [1 ]
Feng, Gang [1 ,2 ,3 ]
Reames, Davis L. [4 ]
Shimer, Adam L. [1 ]
Shen, Francis H. [1 ]
Li, Xudong [1 ]
机构
[1] Univ Virginia, Sch Med, Orthoped Res Labs, Dept Orthoped Surg, Charlottesville, VA 22908 USA
[2] North Sichuan Med Coll, Nanchong Cent Hosp, Dept Orthoped Surg, Nanchong 637000, Peoples R China
[3] North Sichuan Med Coll, Clin Inst 2, Nanchong 637000, Peoples R China
[4] Univ Virginia Hlth Syst, Dept Neurosurg, Charlottesville, VA 22908 USA
来源
SPINE JOURNAL | 2013年 / 13卷 / 03期
关键词
Intervertebral disc; Microgravity; Disc degeneration; Apoptosis; Extracellular matrix; NUCLEUS PULPOSUS; ORGAN-CULTURE; EXTRACELLULAR-MATRIX; LIMITED NUTRITION; BONE-FORMATION; END-PLATE; BACK-PAIN; CELLS; SYSTEM; GROWTH;
D O I
10.1016/j.spinee.2012.01.022
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
BACKGROUND CONTEXT: Astronauts experience back pain, particularly low back pain, during and after spaceflight. Recent studies have described histologic and biochemical changes in rat intervertebral discs after space travel, but there is still no in vitro model to investigate the effects of microgravity on disc metabolism. PURPOSE: To study the effects of microgravity on disc degeneration and establish an in vitro simulated microgravity study model. STUDY DESIGN: Discs were cultured in static and rotating conditions in bioreactor, and the characteristics of disc degeneration were evaluated. METHODS: The mice discs were cultured in a rotating wall vessel bioreactor where the microgravity condition was simulated. Intervertebral discs were cultured in static and microgravity condition. Histology, biochemistry, and immunohistochemical assays were performed to evaluate the characteristics of the discs in microgravity condition. RESULTS: Intervertebral discs cultured in rotating bioreactors were found to develop changes of disc degeneration manifested by reduced red Safranin-O staining within the annulus fibrosus, down-regulated glycosaminoglycan (GAG) content and GAG/hydroxyproline ratio, increased matrix metalloproteinase 3 expression, and upregulated apoptosis. CONCLUSIONS: We conclude that simulated microgravity induces the molecular changes of disc degeneration. The rotating bioreactor model will provide a foundation to investigate the effects of microgravity on disc metabolism. Published by Elsevier Inc.
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页码:235 / 242
页数:8
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