Cell surface shaving of Candida albicans biofilms, hyphae, and yeast form cells

被引:43
|
作者
Vialas, Vital [1 ,2 ]
Perumal, Palani [3 ]
Gutierrez, Dolores [4 ]
Ximenez-Embun, Pilar [4 ]
Nombela, Cesar [1 ,2 ]
Gil, Concha [1 ,2 ,4 ]
Chaffin, W. LaJean [3 ]
机构
[1] Univ Complutense Madrid, Fac Pharm, Dept Microbiol 2, E-28040 Madrid, Spain
[2] Ramon y Cajal Inst Hlth Res IRYCIS, E-28040 Madrid, Spain
[3] Texas Tech Univ Hlth Sci Ctr, Dept Microbiol & Immunol, Lubbock, TX USA
[4] UCM Sci Pk, Prote Unit, Madrid, Spain
关键词
Candida albicans; Cell wall; Microbiology; Protein digest; Protein identification; SYSTEMIC CANDIDIASIS; PROTEIN INFORMATION; WALL PROTEINS; IDENTIFICATION; PROTEOMICS; ANTIGENS; GENE;
D O I
10.1002/pmic.201100588
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We used a brief trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS to identify the surface proteins on live Candida albicans organisms growing in biofilms and planktonic yeast cells and hyphae. One hundred thirty-one proteins were present in at least two of the three replicates of one condition and distributed in various combinations of the three growth conditions. Both previously reported and new surface proteins were identified and these were distributed between covalently attached proteins and noncovalently attached proteins of the cell wall.
引用
收藏
页码:2331 / 2339
页数:9
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