Roseomonas aeriglobus sp nov., isolated from an air-conditioning system

A novel pale pink-coloured, strictly aerobic, Gram-stain negative bacterial strain, designated strain KER25-12(T), was isolated from a laboratory air-conditioning system in South Korea. Cells were observed to be non-motile cocci showing positive catalase and oxidase reactions. Strain KER25-12(T) was found to grow at 10-30 A degrees C (optimum, 25-30 A degrees C), at pH 4.0-9.0 (optimum, pH 6.0-7.0) and in the presence of 0-2% (w/v) NaCl (optimum, 0%). Ubiquinone-10 and spermidine were detected as the sole respiratory quinone and the predominant polyamine, respectively. The major fatty acids were identified as summed feature 8 (comprising C-18:1 omega 7c and/or C-18:1 omega 6c), summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c), C-16:0 and C-18:0. The genomic DNA G+C content of strain KER25-12(T) was determined to be 70.0 mol%. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unidentified aminolipid. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain KER25-12(T) belongs to the genus Roseomonas and shows high sequence similarity to Roseomonas aerilata 5420S-30(T) (98.57%), Roseomonas pecuniae N75(T) (97.44%) and Roseomonas vinacea CPCC 100056(T) (97.40%). Based on the morphological, physiological, chemotaxonomic and phylogenetic features, strain KER25-12(T) is concluded to represent a novel species of the genus Roseomonas, for which the name Roseomonas aeriglobus sp. nov. is proposed. The type strain is KER25-12(T) (= KACC 19282(T) = JCM 32049(T)).