A case-control study of genotoxicity endpoints in patients with papillary thyroid cancer

被引：6
作者：
Geric, Marko [1 ]
Janusic, Renato [2 ]
Sarcevic, Boena [3 ]
Garaj-Vrhovac, Vera [1 ]
机构：
[1] Inst Med Res & Occupat Hlth, Mutagenesis Unit, Zagreb 10000, Croatia
[2] Clin Hosp Tumours, Dept Head & Neck Surg, Zagreb, Croatia
[3] Clin Hosp Tumours, Dept Pathol, Zagreb, Croatia
来源：
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2015年 / 784卷
关键词：
Micronucleus test; Comet assay; Papillary thyroid cancer; Cancer prevention; Raf-B; Ret; PERIPHERAL-BLOOD LYMPHOCYTES; BLOCK MICRONUCLEUS ASSAY; HUMN PROJECT; LUNG-CANCER; COMET ASSAY; DNA-DAMAGE; FREQUENCY; RISK; MECHANISMS; EXPRESSION;
D O I：
10.1016/j.mrgentox.2015.05.006
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Thyroid cancer is one of the fastest growing cancer types worldwide. Using the cytokinesis-block micronucleus (CBMN) and comet assays, we performed a case-control study of 23 untreated papillary thyroid cancer (PTC) patients and 23 healthy volunteers. PTC patients showed higher basal DNA damage in peripheral blood lymphocytes. The CBMN assay indicated that the numbers of micronuclei, nuclear buds, and nucleoplasmic bridges among the cases were 2.67-, 2.79-, and 7.72-fold higher, respectively, than among the controls (p<0.05). Comet assay tail lengths and tail intensities were 1.20- and 1.94-fold higher, respectively (p<0.05). In additional, 14 thyroid tissues from PTC patients were probed for Raf-B and Ret expression; all samples were positive for at least one of these proteins. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：47 / 50
页数：4
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