The Role of IgG Avidity in Diagnosis of Cytomegalovirus Infection in Newborns and Infants

被引:3
作者
Vilibic-Cavlek, Tatjana [1 ,4 ]
Ljubin-Sternak, Suncanica [1 ,4 ]
Vojnovic, Gordana [2 ]
Sviben, Mario [3 ,4 ]
Mlinaric-Galinovic, Gordana [1 ,4 ]
机构
[1] Croatian Natl Inst Publ Hlth, Dept Virol, Zagreb 10000, Croatia
[2] Croatian Natl Inst Publ Hlth, Dept Mol Diagnost, Zagreb 10000, Croatia
[3] Croatian Natl Inst Publ Hlth, Dept Parasitol, Zagreb 10000, Croatia
[4] Univ Zagreb, Sch Med, Zagreb 41001, Croatia
关键词
CMW; diagnosis; IgG avidity; newborns; infants; PRIMARY CMV INFECTION;
D O I
暂无
中图分类号
Q98 [人类学];
学科分类号
030303 ;
摘要
To evaluate the value of IgG avidity in diagnosis of congenital cytomegalovirus (CMV) infection in newborns and infants we collected serum samples from 40 infants under 12 months of age with suspected congenital CMV infection. Sera were tested for IgM, IgG and IgG avidity. For 25 of them, virus isolation and/or polymerase chain reaction (PCR) on urine specimens were performed. Thirteen (32.5%) patients showed the presence of CMV IgM antibodies, 3 (7.5%) had equivocal IgM result, and 24(60.0%) patients had IgG antibodies only. Using IgG avidity, CMV infection (low avidity index-AI) was documented in 61.5% IgM positive and 54.2% IgM negative patients. Eight of nine (88.8%) IgM positive patients were positive either on virus isolation or PCR. In IgM negative patients, 46.6% urine cultures were positive for CMV and 66.6% were PCR positive. According to age, IgG avidity demonstrated acute/recent primary CMV infection in 58.8% patients younger than three months compared with 91.7% and 81.8% in 3-6 and 6-12 months old babies, respectively. In conclusion, IgG avidity is useful in diagnosis of CMV infection either in IgM positive or IgM negative children older than 3 months of age. In infants less than 3 months, transplacentally derived maternal IgG antibodies of high avidity influence on the IgG avidity result. In these children, CMV infection should be confirmed by direct virologic methods such as virus isolation or PCR.
引用
收藏
页码:297 / 300
页数:4
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