Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts

被引：39

作者：

An, In-Sook ^{[2
]}

An, Sungkwan ^{[1
,2
]}

Kang, Sang-Mo ^{[3
]}

Choe, Tae-Boo ^{[3
]}

Lee, Sung Nae ^{[4
]}

Jang, Hyun Hee ^{[5
]}

Bae, Seunghee ^{[1
]}

机构：

[1] Konkuk Univ, Mol Targeted Drug Res Ctr, Seoul 143701, South Korea

[2] Konkuk Univ, Korea Inst Skin & Clin Sci, Seoul 143701, South Korea

[3] Konkuk Univ, Dept Microbial Engn, Seoul 143701, South Korea

[4] Kyung In Womens Coll, Dept Cosmetol, Inchon 407740, South Korea

[5] Kyungbok Univ, Sch Cosmetol, Pocheon Si 487717, Gyeonggi Do, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2012年 / 30卷 / 05期

关键词：

Centella asiatica; dermal fibroblast; UVB irradiation; miRNA expression; cell viability; ACTIVATED PROTEIN-KINASE; HUMAN KERATINOCYTES; C-JUN; INFLAMMATORY RESPONSES; GTPASE RAC1; RHO-GTPASES; CELL-DEATH; APOPTOSIS; SKIN; JNK;

D O I：

10.3892/ijmm.2012.1117

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The titrated extract of Centella asiatica (TECA) is a reconstituted mixture comprising of asiatic acid, madecassic acid, asiaticoside and madecassoside, and is used as a therapeutic agent in wound healing and also as an anti-microbial, anticancer and anti-aging agent. Although these properties and the associated cell signaling pathways have been elucidated, the cellular mechanism of anti-photoaging upon ultraviolet (UV) exposure in normal human dermal fibroblasts (NHDFs) remains unknown. In this study, we investigated the photoprotective role of TECA via microRNA (miRNA) expression profiling analysis. Low dose of TECA did not exhibit toxicity and showed a protective effect against UVB irradiation in NDHFs. miRNA microarray experiments revealed that specific miRNAs were altered by TECA stimulation in UV B-irradiated NHDFs. Functional bioinformatic analysis showed that the putative target genes of the altered miRNAs were associated with the positive regulation of cell proliferation, anti-apoptosis, small GTPase- and Ras-mediated signal transduction and activation of MAPKK. Therefore, these results suggest that TECA may serve as a potential natural chemoprotective agent against UVB-mediated damage in NHDFs through changes in the expression of specific miRNAs.

引用

页码：1194 / 1202

页数：9

共 46 条

[31]

Maquart FX, 1999, EUR J DERMATOL, V9, P289

[32] Toxic effects of ultraviolet radiation on the skin [J].

Matsumura, Y ;

Ananthaswamy, HN .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2004, 195 (03) :298-308

[33] Total Phenolic Compounds, Flavonoids, and Radical Scavenging Activity of 21 Selected Tropical Plants [J].

Mustafa, R. A. ;

Hamid, A. Abdul ;

Mohamed, S. ;

Abu Bakar, F. .

JOURNAL OF FOOD SCIENCE, 2010, 75 (01) :C28-C35

[34] The UV response of the skin: a review of the MAPK, NFκB and TNFα signal transduction pathways [J].

Muthusamy, Visalini ;

Piva, Terrence J. .

ARCHIVES OF DERMATOLOGICAL RESEARCH, 2010, 302 (01) :5-17

[35] The small Rho GTPase Rac1 controls normal human dermal fibroblasts proliferation with phosphorylation of the oncoprotein c-myc [J].

Nikolova, Ekaterina ;

Mitev, Vanio ;

Zhelev, Nikolal ;

Deroanne, Christophe F. ;

Poumay, Yves .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 359 (03) :834-839

[36] Comprehensive thermodynamic analysis of 3′ double-nucleotide overhangs neighboring Watson-Crick terminal base pairs [J].

O'Toole, Amanda S. ;

Miller, Stacy ;

Haines, Nathan ;

Zink, M. Coleen ;

Serra, Martin J. .

NUCLEIC ACIDS RESEARCH, 2006, 34 (11) :3338-3344

[37]

Ott T, 2002, FEMS MICROBIOL ECOL, V42, P359, DOI 10.1111/j.1574-6941.2002.tb01025.x

[38] Repression of protein synthesis by miRNAs: how many mechanisms? [J].

Pillai, Ramesh S. ;

Bhattacharyya, Suvendra N. ;

Filipowicz, Witold .

TRENDS IN CELL BIOLOGY, 2007, 17 (03) :118-126

[39] The MEKK-JNK pathway is stimulated by alpha(1)-adrenergic receptor and Ras activation and is associated with in vitro and in vivo cardiac hypertrophy [J].

Ramirez, MT ;

Sah, VP ;

Zhao, XL ;

Hunter, JJ ;

Chien, KR ;

Brown, JH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (22) :14057-14061

[40] Trivalent chromium activates Rac-1 and Src and induces switch in the cell death mode in human dermal fibroblasts [J].

Rudolf, Emil ;

Cervinka, Miroslav .

TOXICOLOGY LETTERS, 2009, 188 (03) :236-242

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