Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts

被引：38
作者：
An, In-Sook [2 ]
An, Sungkwan [1 ,2 ]
Kang, Sang-Mo [3 ]
Choe, Tae-Boo [3 ]
Lee, Sung Nae [4 ]
Jang, Hyun Hee [5 ]
Bae, Seunghee [1 ]
机构：
[1] Konkuk Univ, Mol Targeted Drug Res Ctr, Seoul 143701, South Korea
[2] Konkuk Univ, Korea Inst Skin & Clin Sci, Seoul 143701, South Korea
[3] Konkuk Univ, Dept Microbial Engn, Seoul 143701, South Korea
[4] Kyung In Womens Coll, Dept Cosmetol, Inchon 407740, South Korea
[5] Kyungbok Univ, Sch Cosmetol, Pocheon Si 487717, Gyeonggi Do, South Korea
来源：
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2012年 / 30卷 / 05期
关键词：
Centella asiatica; dermal fibroblast; UVB irradiation; miRNA expression; cell viability; ACTIVATED PROTEIN-KINASE; HUMAN KERATINOCYTES; C-JUN; INFLAMMATORY RESPONSES; GTPASE RAC1; RHO-GTPASES; CELL-DEATH; APOPTOSIS; SKIN; JNK;
D O I：
10.3892/ijmm.2012.1117
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The titrated extract of Centella asiatica (TECA) is a reconstituted mixture comprising of asiatic acid, madecassic acid, asiaticoside and madecassoside, and is used as a therapeutic agent in wound healing and also as an anti-microbial, anticancer and anti-aging agent. Although these properties and the associated cell signaling pathways have been elucidated, the cellular mechanism of anti-photoaging upon ultraviolet (UV) exposure in normal human dermal fibroblasts (NHDFs) remains unknown. In this study, we investigated the photoprotective role of TECA via microRNA (miRNA) expression profiling analysis. Low dose of TECA did not exhibit toxicity and showed a protective effect against UVB irradiation in NDHFs. miRNA microarray experiments revealed that specific miRNAs were altered by TECA stimulation in UV B-irradiated NHDFs. Functional bioinformatic analysis showed that the putative target genes of the altered miRNAs were associated with the positive regulation of cell proliferation, anti-apoptosis, small GTPase- and Ras-mediated signal transduction and activation of MAPKK. Therefore, these results suggest that TECA may serve as a potential natural chemoprotective agent against UVB-mediated damage in NHDFs through changes in the expression of specific miRNAs.
引用
收藏
页码：1194 / 1202
页数：9
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