Characterization and pathogenicity of Colletotrichum spp. causing citrus anthracnose in Tunisia

被引:0
作者
Daoud, Haifa Ben Hadj [1 ]
Baraldi, Elena [2 ]
Iotti, Mirco [3 ]
Leonardi, Pamela [2 ]
Boughalleb-M'Hamdi, Naima [1 ]
机构
[1] Univ Sousse, High Inst Agron Chott Mariem, Dept Biol Sci & Plant Protect, UR13AGR03, Sousse 4042, Tunisia
[2] Univ Bologna, DISTAL, Dept Agr & Food Sci, Via le Fanin 46, I-40127 Bologna, Italy
[3] Univ LAquila, Dept Life Hlth & Environm Sci, Via Vetoio,Coppito 1, I-67100 Laquila, Italy
关键词
Citrus anthracnose; Colletotrichum karstii; Colletotrichum gloeosporioides; PCR; POSTBLOOM FRUIT DROP; KEY LIME ANTHRACNOSE; 1ST REPORT; MOLECULAR CHARACTERIZATION; CAUSAL AGENT; C; KARSTII; GLOEOSPORIOIDES; DISEASE; CROPS; STRAINS;
D O I
10.13128/Phytopathol_Mediterr-23762
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
In the winter and spring of 2014-2015, typical anthracnose symptoms were detected on different citrus varieties in Cap-Bon and Morneg-Tunis regions of Northern Tunisia. Surveys were conducted to determine the casual agents of these symptoms. A total of seven monosporic isolates were obtained from dark lesions on fruits, flowers, leaves and twigs of citrus from six orchards. One Colletotrichum karstii (from the C. boninense species complex) and six C. gloeosporioides isolates were identified through morphological analysis and sequencing of their ITS rDNA sequences. Pathogenicity tests with the seven isolates were performed on symptomless, detached citrus fruits (Valencia orange and Eureka lemon). All tested isolates caused anthracnose lesions after 1 week of incubation. Koch's postulates were fulfilled by re-isolation of pathogens from the inoculated fruits. This report elucidates the diversity of anthracnose pathogens in Tunisia. This is the first report of C. karstii from citrus in Tunisia.
引用
收藏
页码:175 / 185
页数:11
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