Tumour-associated macrophages: Relation with progression and invasiveness, and assessment of M1/M2 macrophages in canine mammary tumours

被引:39
作者
Monteiro, L. N. [1 ]
Rodrigues, M. A. [1 ,2 ]
Gomes, D. A. [2 ]
Salgado, B. S. [3 ]
Cassali, G. D. [1 ]
机构
[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Patol Geral, Lab Patol Comparada, BR-31270091 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Lab Imunol Celular & Mol, BR-31270091 Belo Horizonte, MG, Brazil
[3] Univ Fed Espirito Santo, Dept Patol, Ctr Ciencias Saude, Lab Imunopatol Life, BR-29043900 Vitoria, Brazil
关键词
Canine; Inflammation; Mammary tumour; Tumour-associated macrophages; Tumour microenvironment; CELL MIGRATION; DIVERSITY; POLARIZATION; CARCINOMAS; BREAST; MODEL;
D O I
10.1016/j.tvjl.2018.02.016
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Macrophages represent a major component of the overall leucocyte population within neoplasms and are important for tumour behaviour in several cancers in human beings. However, little information regarding their role in canine mammary tumours (CMTs) is available. The aim of this study was to address the potential role of tumour-associated macrophages (TAMs) in CMTs. TAMs in CMTs excised from 82 female dogs were quantified at high power (400x magnification) and categorised as low (<= 50) or high (>50) TAM counts. Higher TAM counts were associated with clinical stage (139 0.001), tumour type (P=0.016), tumour size (P=0.013), vascular invasion (P=0.031), lymph node metastasis (P=0.003), high proliferation rates (P=0.009), vascular microdensity (P=0.008), invasive tumour profile (P=0.002) and worse prognosis (P=0.018; hazard ratio = 0.283). Almost all macrophages infiltrating malignant tumours with high TAM counts expressed CD206 (macrophage mannose receptor 1), while all benign tumours were infiltrated by macrophages expressing inducible nitric oxide synthase (NOS2), suggesting a phenotypic shift from classically activated macrophage (M1) subpopulations towards alternatively activated macrophage (M2) subpopulations in malignant tumours. A triple staining pattern revealed mixed M1/M2 profiles in some tumours, thus characterising an intermediate state. The results indicate that TAMs are associated with more aggressive types of mammary cancer in dogs. (C) 2018 Elsevier Ltd. All rights reserved.
引用
收藏
页码:119 / 125
页数:7
相关论文
共 50 条
[21]   Modulators of the Balance between M1 and M2 Macrophages during Pregnancy [J].
Zhang, Yong-Hong ;
He, Ming ;
Wang, Yan ;
Liao, Ai-Hua .
FRONTIERS IN IMMUNOLOGY, 2017, 8
[22]   Regulatory Mechanism of lncRNAs in M1/M2 Macrophages Polarization in the Diseases of Different Etiology [J].
Jiang, Ping ;
Li, Xiaopeng .
FRONTIERS IN IMMUNOLOGY, 2022, 13
[23]   Rabbit M1 and M2 macrophages can be induced by human recombinant GM-CSF and M-CSF [J].
Yamane, Kazuyoshi ;
Leung, Kai-Poon .
FEBS OPEN BIO, 2016, 6 (09) :945-953
[24]   Molecular model linking Th2 polarized M2 tumour-associated macrophages with deaminase-mediated cancer progression mutation signatures [J].
Mamrot, Jared ;
Balachandran, Siddharth ;
Steele, Edward J. ;
Lindley, Robyn A. .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2019, 89 (05)
[25]   Activated macrophages release microvesicles containing polarized M1 or M2 mRNAs [J].
Garzetti, Livia ;
Menon, Ramesh ;
Finardi, Annamaria ;
Bergami, Alessandra ;
Sica, Antonio ;
Martino, Gianvito ;
Comi, Giancarlo ;
Verderio, Claudia ;
Farina, Cinthia ;
Furlan, Roberto .
JOURNAL OF LEUKOCYTE BIOLOGY, 2014, 95 (05) :817-825
[26]   Eicosanoid production by macrophages during inflammation depends on the M1/M2 phenotype [J].
Cui, Jing ;
Shan, Kai ;
Yang, Qin ;
Chen, Wei ;
Feng, Ninghan ;
Chen, Yong Q. .
PROSTAGLANDINS & OTHER LIPID MEDIATORS, 2022, 160
[27]   Viperin Deficiency Promotes Polarization of Macrophages and Secretion of M1 and M2 Cytokines [J].
Eom, John ;
Yoo, Jihye ;
Kim, Jeong Jin ;
Lee, Jae Bong ;
Choi, Wanho ;
Park, Chae Gyu ;
Seo, Jun-Young .
IMMUNE NETWORK, 2018, 18 (04)
[28]   Distribution of M1 and M2 macrophages in cerebral granulomas caused by Encephalitozoon cuniculi [J].
Soto-Dominguez, Adolfo ;
Gonzalez-Castillo, Jennifer A. ;
Castillo-Velazquez, Uziel ;
Rodriguez-Tovar, Luis E. ;
Mendez-Zamora, Gerardo ;
Zamora-Avila, Diana E. ;
Nevarez-Garza, Alicia M. .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2022, 252
[29]   Imbalance of M1/M2 macrophages is linked to severity level of knee osteoarthritis [J].
Liu, Baolong ;
Zhang, Maoquan ;
Zhao, Jingming ;
Zheng, Mei ;
Yang, Hao .
EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2018, 16 (06) :5009-5014
[30]   Chitooligosaccharides alleviate hepatic fibrosis by regulating the polarization of M1 and M2 macrophages [J].
Liu, Peng ;
Li, Heng ;
Gong, Jinsong ;
Geng, Yan ;
Jiang, Min ;
Xu, Hongyu ;
Xu, Zhenghong ;
Shi, Jinsong .
FOOD & FUNCTION, 2022, 13 (02) :753-768