Sensitivity enhancement of SPR biosensor with silver mirror reaction on the Ag/Au film

被引：51

作者：

Wang, Liying ^{[1
]}

Sun, Ying ^{[1
]}

Wang, Jian ^{[1
]}

Zhu, Xiaonan ^{[1
]}

Jia, Fei ^{[1
]}

Cao, Yanbo ^{[1
]}

Wang, Xinghua ^{[1
]}

Zhang, Hanqi ^{[1
]}

Song, Daqian ^{[1
]}

机构：

[1] Jilin Univ, Coll Chem, Changchun 130012, Peoples R China

来源：

TALANTA | 2009年 / 78卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Wavelength modulation; Surface plasmon resonance; Silver mirror reaction; Ag/Au film; SURFACE-PLASMON RESONANCE; SIMPLE CHEMICAL METHOD; SENSORS; IMMUNOSENSOR; ANTIBODY; BINDING; MATRIX; PROBE; DNA; AU;

D O I：

10.1016/j.talanta.2008.11.019

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Based on well-known silver mirror reaction the Ag film was formed on Au film modified by self-assembled monolayer (SAM) of 1,6-hexanedithiol (HDT). The sensitivity of the biosensor based on this Ag/Au film is enhanced compared to that based on Au film. When the surface plasmon resonance (SPR) biosensor based on this Ag/Au film was used to determine human IgG, the range of concentrations of human IgG that could be determined is 0.30-40.00 mu g mL(-1). The lowest concentration (0.30 mu g mL(-1)) that could be detected was about 8 times lower than that obtained by the biosensor without modification by Ag film (2.50 mu g mL(-1)), which demonstrated that the biosensor based on Ag/Au flim could make the resonant wavelength move to longer wavelength following with the sensitivity enhancement of the SPR biosensor. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：265 / 269

页数：5

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