Sensitivity enhancement of SPR biosensor with silver mirror reaction on the Ag/Au film

被引:49
作者
Wang, Liying [1 ]
Sun, Ying [1 ]
Wang, Jian [1 ]
Zhu, Xiaonan [1 ]
Jia, Fei [1 ]
Cao, Yanbo [1 ]
Wang, Xinghua [1 ]
Zhang, Hanqi [1 ]
Song, Daqian [1 ]
机构
[1] Jilin Univ, Coll Chem, Changchun 130012, Peoples R China
基金
中国国家自然科学基金;
关键词
Wavelength modulation; Surface plasmon resonance; Silver mirror reaction; Ag/Au film; SURFACE-PLASMON RESONANCE; SIMPLE CHEMICAL METHOD; SENSORS; IMMUNOSENSOR; ANTIBODY; BINDING; MATRIX; PROBE; DNA; AU;
D O I
10.1016/j.talanta.2008.11.019
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Based on well-known silver mirror reaction the Ag film was formed on Au film modified by self-assembled monolayer (SAM) of 1,6-hexanedithiol (HDT). The sensitivity of the biosensor based on this Ag/Au film is enhanced compared to that based on Au film. When the surface plasmon resonance (SPR) biosensor based on this Ag/Au film was used to determine human IgG, the range of concentrations of human IgG that could be determined is 0.30-40.00 mu g mL(-1). The lowest concentration (0.30 mu g mL(-1)) that could be detected was about 8 times lower than that obtained by the biosensor without modification by Ag film (2.50 mu g mL(-1)), which demonstrated that the biosensor based on Ag/Au flim could make the resonant wavelength move to longer wavelength following with the sensitivity enhancement of the SPR biosensor. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:265 / 269
页数:5
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