共 31 条
Sensitivity enhancement of SPR biosensor with silver mirror reaction on the Ag/Au film
被引:49
作者:
Wang, Liying
[1
]
Sun, Ying
[1
]
Wang, Jian
[1
]
Zhu, Xiaonan
[1
]
Jia, Fei
[1
]
Cao, Yanbo
[1
]
Wang, Xinghua
[1
]
Zhang, Hanqi
[1
]
Song, Daqian
[1
]
机构:
[1] Jilin Univ, Coll Chem, Changchun 130012, Peoples R China
来源:
基金:
中国国家自然科学基金;
关键词:
Wavelength modulation;
Surface plasmon resonance;
Silver mirror reaction;
Ag/Au film;
SURFACE-PLASMON RESONANCE;
SIMPLE CHEMICAL METHOD;
SENSORS;
IMMUNOSENSOR;
ANTIBODY;
BINDING;
MATRIX;
PROBE;
DNA;
AU;
D O I:
10.1016/j.talanta.2008.11.019
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
Based on well-known silver mirror reaction the Ag film was formed on Au film modified by self-assembled monolayer (SAM) of 1,6-hexanedithiol (HDT). The sensitivity of the biosensor based on this Ag/Au film is enhanced compared to that based on Au film. When the surface plasmon resonance (SPR) biosensor based on this Ag/Au film was used to determine human IgG, the range of concentrations of human IgG that could be determined is 0.30-40.00 mu g mL(-1). The lowest concentration (0.30 mu g mL(-1)) that could be detected was about 8 times lower than that obtained by the biosensor without modification by Ag film (2.50 mu g mL(-1)), which demonstrated that the biosensor based on Ag/Au flim could make the resonant wavelength move to longer wavelength following with the sensitivity enhancement of the SPR biosensor. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:265 / 269
页数:5
相关论文