Genetic Aspects of Cell Line Development from a Synthetic Biology Perspective

被引：4
作者：
Botezatu, L. [1 ]
Sievers, S. [1 ]
Gama-Norton, L. [1 ]
Schucht, R. [1 ]
Hauser, H. [1 ]
Wirth, D. [1 ]
机构：
[1] Helmholtz Ctr Infect Res, Braunschweig, Germany
来源：
GENOMICS AND SYSTEMS BIOLOGY OF MAMMALIAN CELL CULTURE | 2012年 / 127卷
关键词：
Synthetic biology; Mammalian cells; Controlled genetic engineering; Synthetic expression cassettes; Controlled intervention; HAMSTER OVARY CELLS; LOW CULTURE TEMPERATURE; INACTIVATING RETROVIRAL VECTORS; PROTEIN DISULFIDE-ISOMERASE; CRE RECOMBINASE ACTIVITY; LARGE T-ANTIGEN; MAMMALIAN-CELLS; TRANSGENE EXPRESSION; DOWN-REGULATION; REVERSIBLE IMMORTALIZATION;
D O I：
10.1007/10_2011_117
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Animal cells can be regarded as factories for the production of relevant proteins. The advances described in this chapter towards the development of cell lines with higher productivity capacities, certain metabolic and proliferation properties, reduced apoptosis and other features must be regarded in an integrative perspective. The systematic application of systems biology approaches in combination with a synthetic arsenal for targeted modification of endogenous networks are proposed to lead towards the achievement of a predictable and technologically advanced cell system with high biotechnological impact.
引用
收藏
页码：251 / 284
页数：34
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