Genetic Aspects of Cell Line Development from a Synthetic Biology Perspective

被引：4
作者：
Botezatu, L. [1 ]
Sievers, S. [1 ]
Gama-Norton, L. [1 ]
Schucht, R. [1 ]
Hauser, H. [1 ]
Wirth, D. [1 ]
机构：
[1] Helmholtz Ctr Infect Res, Braunschweig, Germany
来源：
GENOMICS AND SYSTEMS BIOLOGY OF MAMMALIAN CELL CULTURE | 2012年 / 127卷
关键词：
Synthetic biology; Mammalian cells; Controlled genetic engineering; Synthetic expression cassettes; Controlled intervention; HAMSTER OVARY CELLS; LOW CULTURE TEMPERATURE; INACTIVATING RETROVIRAL VECTORS; PROTEIN DISULFIDE-ISOMERASE; CRE RECOMBINASE ACTIVITY; LARGE T-ANTIGEN; MAMMALIAN-CELLS; TRANSGENE EXPRESSION; DOWN-REGULATION; REVERSIBLE IMMORTALIZATION;
D O I：
10.1007/10_2011_117
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Animal cells can be regarded as factories for the production of relevant proteins. The advances described in this chapter towards the development of cell lines with higher productivity capacities, certain metabolic and proliferation properties, reduced apoptosis and other features must be regarded in an integrative perspective. The systematic application of systems biology approaches in combination with a synthetic arsenal for targeted modification of endogenous networks are proposed to lead towards the achievement of a predictable and technologically advanced cell system with high biotechnological impact.
引用
收藏
页码：251 / 284
页数：34
相关论文
共 215 条
  • [1] Second-generation tetracycline-regulatable promoter: repositioned tet operator elements optimize transactivator synergy while shorter minimal promoter offers tight basal leakiness
    Agha-Mohammadi, S
    O'Malley, M
    Etemad, A
    Wang, Z
    Xiao, X
    Lotze, MT
    [J]. JOURNAL OF GENE MEDICINE, 2004, 6 (07) : 817 - 828
  • [2] Ailor E, 1998, BIOTECHNOL BIOENG, V58, P196, DOI 10.1002/(SICI)1097-0290(19980420)58:2/3<196::AID-BIT12>3.3.CO
  • [3] 2-0
  • [4] A novel tetracycline-dependent transactivator with E2F4 transcriptional activation domain
    Akagi, Kiwamu
    Kanai, Masayuki
    Saya, Hideyuki
    Kozu, Tomoko
    Berns, Anton
    [J]. NUCLEIC ACIDS RESEARCH, 2001, 29 (04) : 23
  • [5] Cellular transformation by SV40 large T antigen: interaction with host proteins
    Ali, SH
    DeCaprio, JA
    [J]. SEMINARS IN CANCER BIOLOGY, 2001, 11 (01) : 15 - 22
  • [6] Life and death in mammalian cell culture: strategies for apoptosis inhibition
    Arden, N
    Betenbaugh, MJ
    [J]. TRENDS IN BIOTECHNOLOGY, 2004, 22 (04) : 174 - 180
  • [7] Mammalian synthetic biology - from tools to therapies
    Aubel, Dominique
    Fussenegger, Martin
    [J]. BIOESSAYS, 2010, 32 (04) : 332 - 345
  • [8] Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein-Barr virus replication and the simple tetracycline repressor
    Bach, Markus
    Grigat, Silke
    Pawlik, Barbara
    Fork, Christian
    Utermoehlen, Olaf
    Pal, Sonia
    Banczyk, David
    Lazar, Andreas
    Schoemig, Edgar
    Gruendemann, Dirk
    [J]. FEBS JOURNAL, 2007, 274 (03) : 783 - 790
  • [9] Chemical control of protein stability and function in living mice
    Banaszynski, Laura A.
    Sellmyer, Mark A.
    Contag, Christopher H.
    Wandless, Thomas J.
    Thorne, Steve H.
    [J]. NATURE MEDICINE, 2008, 14 (10) : 1123 - 1127
  • [10] Tetracycline-controlled transcription in eukaryotes: Novel transactivators with graded transactivation potential
    Baron, U
    Gossen, M
    Bujard, H
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (14) : 2723 - 2729
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