Effect of recombinant serine protease from adult stage of Trichinella spiralis on TNBS-induced experimental colitis in mice

被引：26

作者：

Pang, Jianda ^{[1
]}

Ding, Jing ^{[1
]}

Zhang, Lixiao ^{[1
]}

Zhang, Yuanyuan ^{[1
]}

Yang, Yaming ^{[2
]}

Bai, Xue ^{[1
]}

Liu, Xiaolei ^{[1
]}

Jin, Xuemin ^{[1
]}

Guo, Heng ^{[3
]}

Yang, Yong ^{[1
]}

Liu, Mingyuan ^{[1
,4
]}

机构：

[1] Jilin Univ, Coll Vet Med, Inst Zoonosis, Key Lab Zoonosis Res,Minist Educ, Changchun 130062, Peoples R China

[2] Yunnan Inst Parasit Dis, 6 Xiyuan Rd, Puer, Yunnan, Peoples R China

[3] Beijing Hitech Inst, Beijing 100094, Peoples R China

[4] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225000, Jiangsu, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2020年 / 86卷

基金：

中国国家自然科学基金;

关键词：

Trichinella spiralis; Adult serine protease; TNBS-induced colitis; Th2; Regulatory T cell; INTESTINAL NEMATODE INFECTION; INFLAMMATORY-BOWEL-DISEASE; REGULATORY T-CELLS; HELMINTH THERAPY; ELICITS;

D O I：

10.1016/j.intimp.2020.106699

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Inflammatory bowel disease (IBD), which includes ulcerative colitis (UC) and Crohn's disease (CD), is a chronic autoimmune disease. At present, worms and their products has been shown to have protective effects on immune-mediated diseases. Therefore, we aimed to investigate the effect of the recombination Trichinella spiralis (T. spiralis, Ts) adult serine protease-like protein rTs-ADSp-7 on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced CD mouse model. Colitis was induced by intrarectal administration of a TNBS solution. The disease activity index (DAI), which included weight loss, diarrhoea, and bloody stool, was measured. Colon segments were stained with haematoxylin and eosin (H.E.) for histopathological score. Cytokine release in the serum was analysed by meso scale discovery (MSD). Cytokine release in the colon was detected by ELISA. Splenocytes were separated, and the cytokine profiles of Th1 (IFN-gamma), Th2 (IL-4), Th17 (IL-17A) and Treg cells were analysed by flow cytometry. Our result showed that rTs-ADSp-7 reduced the clinical disease activity of TNBS-induced colitis in mice. In addition, we found that rTs-ADSp-7 reduced the production of Th1- and Th17-related cytokines while upregulating the expression of Th2- and Treg-related cytokines in TNBS-induced colitis mice. rTs-ADSp-7 also increased the population of Th2 and Treg cells in TNBS-induced colitis mice. rTs-ADSp-7 alleviated the severity of TNBS-induced colitis while balancing the CD4(+) T cell immune response. rTs-ADSp-7 has therapeutic potential for colitis treatment and can be used as a helminth-derived protein therapy for CD or other Th1 immunity-mediated diseases.

引用

页数：7

共 33 条

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