Fatty Acid Profiling During Microbial Lipid Production Under Varying pO2 and Impeller Tip Speeds

被引:14
作者
Ahmed, Syed Ubaid [1 ]
Singh, Sudheer Kumar [1 ]
Pandey, Ashok [1 ]
Kanjilal, Sanjit [2 ]
Prasad, Rachapudi B. N. [2 ]
机构
[1] CSIR, Natl Inst Interdisciplinary Sci & Technol, Div Biotechnol, Trivandrum, Kerala, India
[2] Indian Inst Chem Technol, Oils & Fats Div, Hyderabad 500007, Andhra Pradesh, India
关键词
Microbial lipid; Fatty acid profile; Central composite design; Impeller tip speed; Dissolved oxygen concentration;
D O I
10.1007/s12010-008-8261-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The fatty acid profile study was undertaken to study the effect of impeller tip speed-associated shear stress and dissolved oxygen saturation (DO) on the fatty acid composition variation and on total lipid content of the cells. The study was undertaken in a 5-l stirred tank bioreactor using Mucor sp. RRL001. To study the interaction of parameters and their effects, a central composite design was used. The fatty acid profiling during the course of study suggested that oleic acid and palmitic acid were two major components with their composition varying between 34-47% and 29-39.1%, respectively, of the total lipid content. The GLA content varied between 3% and 9% of the total lipid. The lipid profile study also revealed the presence of a minor amount of fatty acids of chain length C:12, C:20, C:22, and C:24. The modeling of lipid accumulation suggested that it follows a quadratic model with both impeller tip speed (p=0.0166) and dissolved oxygen concentration (p=0.0098) following the quadratic order of effect. The fermenter run based on the optimum production zone in response surface plot resulted in the maximum 4.8 g l(-1) lipid compared with the model-predicted value of 4.49 g l(-1). The present study suggests that dissolved oxygen saturation is a more significant contributor to total lipid accumulation. However, the study also suggests that the fatty acid profile of fungal lipid is not directly associated with the shear stress or oxygen availability in Mucor sp. RRL001.
引用
收藏
页码:599 / 609
页数:11
相关论文
共 29 条
[1]  
Ahmed SU, 2006, FOOD TECHNOL BIOTECH, V44, P283
[2]  
AMANO N, 1992, MYCOTAXON, V44, P257
[3]   BIOMODIFICATION OF FATS AND OILS - TRIALS WITH CANDIDA-LIPOLYTICA [J].
BATI, N ;
HAMMOND, EG ;
GLATZ, BA .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1984, 61 (11) :1743-1746
[4]  
Box G., 2005, Statistics for Experimenters, VSecond
[5]  
Bruns R. E., 2006, Statistical Design-Chemometrics
[6]  
Certík M, 2006, FOOD TECHNOL BIOTECH, V44, P75
[7]   Lipid formation and gamma-linolenic acid production by Mucorales fungi grown on sunflower oil [J].
Certik, M ;
Balteszova, L ;
Sajbidor, J .
LETTERS IN APPLIED MICROBIOLOGY, 1997, 25 (02) :101-105
[8]   Production of gamma-linolenic acid by the fungus Cunninghamella echinulata CCRC 31840 [J].
Chen, HC ;
Chang, CC .
BIOTECHNOLOGY PROGRESS, 1996, 12 (03) :338-341
[9]   Inoculum effects on the production of gamma-linolenic acid by the shake culture of Cunninghamella echinulata CCRC 31840 [J].
Chen, HC ;
Liu, TM .
ENZYME AND MICROBIAL TECHNOLOGY, 1997, 21 (02) :137-142
[10]   PRODUCTION OF MICROBIAL LIPID - EFFECTS OF GROWTH-RATE AND OXYGEN ON LIPID-SYNTHESIS AND FATTY-ACID COMPOSITION OF RHODOTORULA-GRACILIS [J].
CHOI, SY ;
RYU, DDY ;
RHEE, JS .
BIOTECHNOLOGY AND BIOENGINEERING, 1982, 24 (05) :1165-1172