Enhanced oil accumulation in tobacco (Nicotiana tabacum L.) leaves by ectopic overexpression of VgDGAT1a for renewable production of biofuels

被引：2
作者：
Gao, Chang-Yong [1 ,2 ]
Mao, Xue [1 ]
Shang, Hong-Qin [2 ]
Li, Fang [1 ]
Li, Run-Zhi [1 ]
机构：
[1] Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Taigu 030801, Shanxi, Peoples R China
[2] Heze Univ, Dept Life Sci, Heze 274015, Shandong, Peoples R China
来源：
CURRENT SCIENCE | 2018年 / 114卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Biofuels; metabolic engineering; plant oil; tobacco leaves; VgDGAT1a gene; EXPRESSION;
D O I：
10.18520/cs/v114/i06/1234-1240
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To increase oil accumulation in the high-biomass vegetative organs of tobacco (Nicotiana tabacum L.) plants for renewable production of biofuels, VgDGAT1a isolated from developing seeds of Vernonia galamensis L. was ectopically overexpressed in tobacco leaves using a constitutive promoter. The transgenic tobacco leaves showed a 3.5-5.0-fold increase in oil content compared to the control, with a maximum increase of 9.2% (DW). The transgenic leaves also showed a substantial change in fatty acid composition, with significant enhancement of linoleic acid (18 : 2) and notable reduction of. -linolenic acid (18 : 3). The overexpression of VgDGAT1a exhibited no deleterious effect on other phenotypes in the tobacco plant. These results will facilitate development of a novel system for lipid metabolic engineering in vegetative organs of plants, as well as provide a platform for the production of biofuels using the vegetative organs of commercial non-food crops.
引用
收藏
页码：1234 / 1240
页数：7
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