Association of a functional intronic polymorphism rs735396 in HNF1A gene with the susceptibility to hepatocellular carcinoma in Han Chinese population

被引:0
作者
Jiang, Ming-Ming [1 ]
Gu, Xing [1 ]
Yang, Juan [1 ]
Wang, Meng-Meng [1 ]
Li, Hui-Ming [1 ]
Fang, Meng [1 ]
Gao, Chun-Fang [1 ]
机构
[1] Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Dept Lab Med, Shanghai, Peoples R China
来源
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2017年 / 10卷 / 01期
基金
中国国家自然科学基金;
关键词
HNF1A; HBV-related HCC; single nucleotide polymorphisms; genotype; susceptibility; TRANSCRIPTION FACTORS; EXPRESSION; LIVER; HNF1-ALPHA; MUTATIONS; ALPHA; IDENTIFICATION; HNF-1-ALPHA; PROGRESSION; HNF-1-BETA;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Single nucleotide polymorphism rs735396 located in HNF1A gene was highly associated with the levels of glycan groups which changed during carcinogenesis and progression of HCC after HBV infection. In present study, we investigated if rs735396 are associated with the susceptibility to HBV infection and HBV-related HCC. Methods: Totally 568 HBV-related HCC patients, 110 HBV carriers without HCC and 380 healthy controls in Han Chinese population were recruited and genotyped using polymerase chain reaction-ligation detection reaction (PCR-LDR) method. To evaluate the effects of rs735396 polymorphisms on transcription of HNF1A, the luciferase activity for allelotype A or G was detected in HepG2.2.15 cells. Results: The genotype distribution and allelic frequencies were significantly different between HBV-related HCC patients and healthy controls (P < 0.05). Also, compared with genotype GG, genotypes AA+GA are connected with a higher risk of HBV-related HCC (Odds Ratio = 1.908; 95% Confidence Interval = 1.390-2.619, P < 0.001). Moreover, luciferase activity for allelotype A was significantly lower than that of allelotype G (P = 0.036). Conclusion: Taken together, we demonstrated that the variation of HNF1A rs735396 influences host susceptibility to HBV-related HCC by altering transcriptional activity of HNF1A.
引用
收藏
页码:671 / 679
页数:9
相关论文
共 34 条
[1]   MEMBERS OF THE CAAT/ENHANCER-BINDING PROTEIN, HEPATOCYTE NUCLEAR FACTOR-I AND NUCLEAR FACTOR-I FAMILIES CAN DIFFERENTIALLY MODULATE THE ACTIVITIES OF THE RAT ALPHA-FETOPROTEIN PROMOTER AND ENHANCER [J].
BOISJOYEUX, B ;
DANAN, JL .
BIOCHEMICAL JOURNAL, 1994, 301 :49-55
[2]   Association of a Low-Frequency Variant in HNF1A With Type 2 Diabetes in a Latino Population [J].
Estrada, Karol ;
Aukrust, Ingvild ;
Bjorkhaug, Lise ;
Burtt, Noel P. ;
Mercader, Josep M. ;
Garcia-Ortiz, Humberto ;
Huerta-Chagoya, Alicia ;
Moreno-Macias, Hortensia ;
Walford, Geoffrey ;
Flannick, Jason ;
Williams, Amy L. ;
Gomez-Vazquez, Maria J. ;
Fernandez-Lopez, Juan C. ;
Martinez-Hernandez, Angelica ;
Centeno-Cruz, Federico ;
Mendoza-Caamal, Elvia ;
Revilla-Monsalve, Cristina ;
Islas-Andrade, Sergio ;
Cordova, Emilio J. ;
Soberon, Xavier ;
Gonzalez-Villalpando, Maria E. ;
Henderson, E. ;
Wilkens, Lynne R. ;
Le Marchand, Loic ;
Arellano-Campos, Olimpia ;
Ordonez-Sanchez, Maria L. ;
Rodriguez-Torres, Maribel ;
Rodriguez-Guillen, Rosario ;
Riba, Laura ;
Najmi, Laeya A. ;
Jacobs, Suzanne B. R. ;
Fennell, Timothy ;
Gabriel, Stacey ;
Fontanillas, Pierre ;
Hanis, Craig L. ;
Lehman, Donna M. ;
Jenkinson, Christopher P. ;
Abboud, Hanna E. ;
Bell, Graeme I. ;
Cortes, Maria L. ;
Boehnke, Michael ;
Gonzalez-Villalpando, Clicerio ;
Orozco, Lorena ;
Haiman, Christopher A. ;
Tusie-Luna, Teresa ;
Aguilar-Salinas, Carlos A. ;
Altshuler, David ;
Njolstad, Pal R. ;
Florez, Jose C. ;
MacArthur, Daniel G. .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2014, 311 (22) :2305-2314
[3]   N-glycan based models improve diagnostic efficacies in hepatitis B virus-related hepatocellular carcinoma [J].
Fang, Meng ;
Zhao, Yun-Peng ;
Zhou, Fei-Guo ;
Lu, Lun-Gen ;
Qi, Peng ;
Wang, Hao ;
Zhou, Kun ;
Sun, Shu-Han ;
Chen, Cui-Ying ;
Gao, Chun-Fang .
INTERNATIONAL JOURNAL OF CANCER, 2010, 127 (01) :148-159
[4]  
Hayashi Y, 1999, J CLIN PATHOL-MOL PA, V52, P19
[5]   The private hepatocyte nuclear factor-1α G319S variant is associated with plasma lipoprotein variation in Canadian Oji-Cree [J].
Hegele, RA ;
Cao, HN ;
Harris, SB ;
Hanley, AJG ;
Zinman, B ;
Connelly, PW .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2000, 20 (01) :217-222
[6]   Genetic Study of Complex Diseases in the Post-GWAS Era [J].
Huang, Qingyang .
JOURNAL OF GENETICS AND GENOMICS, 2015, 42 (03) :87-98
[7]   Polymorphisms in B3GAT1, SLC9A9 and MGAT5 are associated with variation within the human plasma N-glycome of 3533 European adults [J].
Huffman, Jennifer E. ;
Knezevic, Ana ;
Vitart, Veronique ;
Kattla, Jayesh ;
Adamczyk, Barbara ;
Novokmet, Mislav ;
Igl, Wilmar ;
Pucic, Maja ;
Zgaga, Lina ;
Johannson, Asa ;
Redzic, Irma ;
Gornik, Olga ;
Zemunik, Tatijana ;
Polasek, Ozren ;
Kolcic, Ivana ;
Pehlic, Marina ;
Koeleman, Carolien A. M. ;
Campbell, Susan ;
Wild, Sarah H. ;
Hastie, Nicholas D. ;
Campbell, Harry ;
Gyllensten, Ulf ;
Wuhrer, Manfred ;
Wilson, James F. ;
Hayward, Caroline ;
Rudan, Igor ;
Rudd, Pauline M. ;
Wright, Alan F. ;
Lauc, Gordan .
HUMAN MOLECULAR GENETICS, 2011, 20 (24) :5000-5011
[8]   Expression of HNFs and C/EBPα is correlated with immunocytochemical differentiation of cell lines derived from human hepatocellular carcinomas, hepatoblastomas and immortalized hepatocytes [J].
Ishiyama, T ;
Kano, J ;
Minami, Y ;
Iijima, T ;
Morishita, Y ;
Noguchi, M .
CANCER SCIENCE, 2003, 94 (09) :757-763
[9]   Expression of alpha 1,6-fucosyltransferase 8 in hepatitis B virus-related hepatocellular carcinoma influences tumour progression [J].
Ji, Jun ;
Gu, Xing ;
Fang, Meng ;
Zhao, Yunpeng ;
Yi, Changhong ;
Wang, Aihua ;
Gao, Chunfang .
DIGESTIVE AND LIVER DISEASE, 2013, 45 (05) :414-421
[10]   Identification of novel serum biomarkers of hepatocellular carcinoma using glycomic analysis [J].
Kamiyama, Toshiya ;
Yokoo, Hideki ;
Furukawa, Jun-Ichi ;
Kurogochi, Masaki ;
Togashi, Tomoaki ;
Miura, Nobuaki ;
Nakanishi, Kazuaki ;
Kamachi, Hirofumi ;
Kakisaka, Tatsuhiko ;
Tsuruga, Yosuke ;
Fujiyoshi, Masato ;
Taketomi, Akinobu ;
Nishimura, Shin-Ichiro ;
Todo, Satoru .
HEPATOLOGY, 2013, 57 (06) :2314-2325