LABEL-FREE ELECTROCHEMICAL DETECTION OF TETRACYCLINE BY AN APTAMER NANO-BIOSENSOR

被引:31
作者
Zhang, Juankun [1 ]
Wu, Yan [1 ]
Zhang, Binbin [1 ]
Li, Min [1 ]
Jia, Shiru [1 ]
Jiang, Shuhai [1 ]
Zhou, Hao [1 ]
Zhang, Yi [1 ]
Zhang, Chaozheng [1 ]
Turner, Anthony P. F. [2 ]
机构
[1] Tianjin Univ Sci & Technol, Tianjin Key Lab Ind Microbiol, Minist Educ, Coll Biotechnol, Tianjin 300457, Peoples R China
[2] Linkoping Univ, Biosensors & Bioelect Ctr, IFM, Linkoping, Sweden
关键词
Aptamer; Biosensor; Impedance; Nano-porous silicon; Tetracycline; SILICON PHOTONIC CRYSTALS; POROUS-SILICON; MOLECULES; MATRIX; CHIP;
D O I
10.1080/00032719.2012.670784
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel aptamer nano-porous silicon (PS) biosensor was investigated for the rapid determination of tetracyclines. Electrochemical impedance spectroscopy (EIS) was used to analyze the behavior of the sensor. The specific binding of tetracycline to the aptamer biosensor led to a decrease in impedance. The corresponding impedance spectra (Nyquist plots) were obtained when serial concentrations of tetracycline were added into the system. An equivalent electrical circuit was used to fit the impedance data. The linear range of the sensor was 2.1-62.4 nM.
引用
收藏
页码:986 / 992
页数:7
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