Sgk1 enhances RANBP1 transcript levels and decreases taxol sensitivity in RKO colon carcinoma cells

被引：54

作者：

Amato, R. ^{[1
]}

Scumaci, D. ^{[2
]}

D'Antona, L. ^{[1
]}

Iuliano, R. ^{[2
,3
]}

Menniti, M. ^{[1
]}

Di Sanzo, M. ^{[2
]}

Faniello, M. C. ^{[2
]}

Colao, E. ^{[3
]}

Malatesta, P. ^{[3
]}

Zingone, A. ^{[4
]}

Agosti, V. ^{[2
]}

Costanzo, F. S. ^{[2
]}

Mileo, A. M. ^{[5
]}

Paggi, M. G. ^{[5
]}

Lang, F. ^{[6
]}

Cuda, G. ^{[2
]}

Lavia, P. ^{[7
]}

Perrotti, N. ^{[1
,3
]}

机构：

[1] Magna Graecia Univ Catanzaro, Dept Human Hlth, Catanzaro, Italy

[2] Magna Graecia Univ Catanzaro, Dept Expt & Clin Med, Catanzaro, Italy

[3] Univ Hosp, Unit Med Genet & Pathol, Policlin Mater Domini, Catanzaro, Italy

[4] NCI, Multiple Myeloma Sect, Metab Branch, Ctr Canc Res,NIH, Bethesda, MD 20892 USA

[5] Natl Canc Inst Regina Elena, Dept Dev Therapeut Programs, Rome, Italy

[6] Univ Tubingen, Dept Physiol, Tubingen, Germany

[7] Univ Roma La Sapienza, CNR, IBPM Inst Mol Biol & Pathol, I-00185 Rome, Italy

来源：

ONCOGENE | 2013年 / 32卷 / 38期

关键词：

Sgk1; RANBP1; taxol sensitivity; mitotic microtubule stabilization; INDUCIBLE PROTEIN-KINASE; BINDING PROTEIN-1; DOWN-REGULATION; CANCER-CELLS; SERUM; ACTIVATION; PHOSPHORYLATION; IDENTIFICATION; COORDINATION; EXPRESSION;

D O I：

10.1038/onc.2012.470

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The serum- and glucocorticoid-regulated kinase (Sgk1) is essential for hormonal regulation of epithelial sodium channel-mediated sodium transport and is involved in the transduction of growth factor-dependent cell survival and proliferation signals. Growing evidence now points to Sgk1 as a key element in the development and/or progression of human cancer. To gain insight into the mechanisms through which Sgk1 regulates cell proliferation, we adopted a proteomic approach to identify up- or downregulated proteins after Sgk1-specific RNA silencing. Among several proteins, the abundance of which was found to be up- or downregulated upon Sgk1 silencing, we focused our attention of RAN-binding protein 1 (RANBP1), a major effector of the GTPase RAN. We report that Sgk1-dependent regulation of RANBP1 has functional consequences on both mitotic microtubule activity and taxol sensitivity of cancer cells.

引用

页码：4572 / 4578

页数：7

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