Screening for anabolic steroids in doping analysis by liquid chromatography/electrospray ion trap mass spectrometry

被引：53

作者：

Deventer, K. ^{[1
]}

Van Eenoo, P. ^{[1
]}

Delbeke, F. T. ^{[1
]}

机构：

[1] Univ Ghent, Dept Clin Chem Microbiol & Immunol, DoCoLab, B-9052 Zwijnaarde, Belgium

来源：

BIOMEDICAL CHROMATOGRAPHY | 2006年 / 20卷 / 05期

关键词：

anabolic steroids; stanozolol; 3 '-hydroxystanozolol; gestrinone; 17; alpha-trenbolone; doping analysis;

D O I：

10.1002/bmc.579

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast and selective LC/MS/MS method for the screening of four anabolic steroids in human urine has been developed and validated. Liquid-liquid extraction with diethyl ether was applied after enzymatic hydrolysis. Analyses were performed on an ion trap mass spectrometer equipped with electrospray ionisation. MS/MS was applied for all compounds. The analytical run time was 11 min. ne LOD for all compounds varied between 1 and 10 ng/mL. Left-over A samples, which were declared positive by GC/MS for the presence of 3'-hydroxystanozolol, were assessed using the described method. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：429 / 433

页数：5

共 16 条

[1] Testing for natural and synthetic anabolic agents in human urine [J].

Ayotte, C ;

Goudreault, D ;

Charlebois, A .

JOURNAL OF CHROMATOGRAPHY B, 1996, 687 (01) :3-25

[2] Mechanistic aspects of electrospray ionization [J].

Bruins, AP .

JOURNAL OF CHROMATOGRAPHY A, 1998, 794 (1-2) :345-357

[3] Tetrahydrogestrinone: discovery, synthesis, and detection in urine [J].

Catlin, DH ;

Sekera, MH ;

Ahrens, BD ;

Starcevic, B ;

Chang, YC ;

Hatton, CK .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2004, 18 (12) :1245-1249

[4]

Choi MH, 1999, RAPID COMMUN MASS SP, V13, P376, DOI 10.1002/(SICI)1097-0231(19990315)13:5<376::AID-RCM494>3.0.CO

[5]

2-#

[6]

DEVENTER K, IN PRESS J CHROMAT B

[7]

Eurachem Working Group, 1998, LAB GUID METH VAL RE

[8] Sensitive liquid chromatographic/tandem mass spectrometric method for the determination of beclomethasone dipropionate and its metabolites in equine plasma and urine [J].

Guan, FY ;

Uboh, C ;

Soma, L ;

Hess, A ;

Luo, Y ;

Tsang, DS .

JOURNAL OF MASS SPECTROMETRY, 2003, 38 (08) :823-838

[9] Screening of free 17-alkyl-substituted anabolic steroids in human urine by liquid chromatography-electrospray ionization tandem mass spectrometry [J].

Leinonen, A ;

Kuuranne, T ;

Kotiaho, T ;

Kostiainen, R .

STEROIDS, 2004, 69 (02) :101-109

[10] Liquid chromatography/mass spectrometry in anabolic steroid analysis-optimization and comparison of three ionization techniques: electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photoionization [J].

Leinonen, A ;

Kuuranne, T ;

Kostiainen, R .

JOURNAL OF MASS SPECTROMETRY, 2002, 37 (07) :693-698

← 1 2 →