Regulation of replicon size in Xenopus egg extracts

被引:147
作者
Waiter, J [1 ]
Newport, JW [1 ]
机构
[1] UNIV CALIF SAN DIEGO, DEPT BIOL, LA JOLLA, CA 92093 USA
关键词
D O I
10.1126/science.275.5302.993
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Once a specific number of cells have been produced in the early Xenopus laevis embryo, replicon size during the S phase of the cell cycle increases. Here, it is reported that similar increase in replicon size occurred when the concentration of nuclei in replication-competent Xenopus egg extracts exceeded a critical threshold. In this system, the origin recognition complex (ORC) did not become stoichiometrically limiting for initiation, and similar amounts of this complex bound to chromatin regardless of replicon size. These data suggest that in early development, an unidentified factor controls how many preformed ORC-DNA complexes initiate DNA replication.
引用
收藏
页码:993 / 995
页数:3
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