Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion

被引：14

作者：

Erickson, Bruce ^{[1
,2
]}

Selvan, Senthamil Paramadayalan ^{[1
,2
]}

Ko, Kerry W. S. ^{[1
,2
]}

Kelly, Karen ^{[1
,3
]}

Quiroga, Ariel D. ^{[1
,2
]}

Li, Lena ^{[1
,2
]}

Nelson, Randy ^{[1
,2
]}

King-Jones, Kirst ^{[4
]}

Jacobs, Rene L. ^{[1
,3
]}

Lehner, Richard ^{[1
,2
,5
]}

机构：

[1] Univ Alberta, Mazankowski Alberta Heart Inst, Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada

[2] Univ Alberta, Mazankowski Alberta Heart Inst, Dept Pediat, Edmonton, AB T6G 2S2, Canada

[3] Univ Alberta, Mazankowski Alberta Heart Inst, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2S2, Canada

[4] Univ Alberta, Fac Sci, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada

[5] Univ Alberta, Mazankowski Alberta Heart Inst, Dept Cell Biol, Edmonton, AB T6G 2S2, Canada

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2013年 / 1831卷 / 06期

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

Hepatic lipase; Triacylglycerol; VLDL; apoB; Fatty acid oxidation; Endoplasmic reticulum; TRIGLYCERIDE TRANSFER PROTEIN; FATTY LIVER-DISEASE; APOLIPOPROTEIN-B SECRETION; HIGH-DENSITY-LIPOPROTEINS; CHOLESTERYL ESTERS; SELECTIVE UPTAKE; APO-B; NONALCOHOLIC STEATOHEPATITIS; METABOLISM; HYDROLASE;

D O I：

10.1016/j.bbalip.2013.01.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hepatic triacylglycerol levels are governed through synthesis, degradation and export of this lipid. Here we demonstrate that enforced expression of hepatic lipase in the endoplasmic reticulum in McArdle RH7777 hepatocytes resulted in a significant decrease in the incorporation of fatty acids into cellular triacylglycerol and cholesteryl ester accompanied by attenuation of secretion of apolipoprotein B-containing lipoproteins. Hepatic lipase-mediated depletion of intracellular lipid storage increased the expression of peroxisome proliferator-activated receptor alpha and its target genes and augmented oxidation of fatty acids. These data show that I) hepatic lipase is active in the endoplasmic reticulum and 2) intracellular hepatic lipase modulates cellular lipid metabolism and lipoprotein secretion. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：1113 / 1123

页数：11

共 59 条

[1] SUBCELLULAR-LOCALIZATION OF B APOPROTEIN OF PLASMA LIPOPROTEINS IN RAT-LIVER [J].