Photostable pH-Sensitive Near-Infrared Aggregation-Induced Emission Luminogen for Long-Term Mitochondrial Tracking

被引：61
作者：
Zhao, Xiujie [1 ,2 ]
Chen, Yun [1 ,2 ]
Niu, Guiyu [1 ,2 ]
Gu, Dening [1 ,2 ]
Wang, Jianning [1 ,2 ]
Cao, Yanmei [1 ,2 ]
Yin, Yongmei [1 ,2 ]
Li, Xiaogang [5 ,6 ]
Ding, Dan [3 ,4 ]
Xi, Rimo [1 ,2 ]
Meng, Meng [1 ,2 ]
机构：
[1] Nankai Univ, State Key Lab Med Chem Biol, Coll Pharm, Tianjin 300353, Peoples R China
[2] Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300353, Peoples R China
[3] Nankai Univ, Minist Educ, Key Lab Bioact Mat, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
[4] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
[5] Peking Union Med Coll, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China
[6] Chinese Acad Med Sci, Beijing 100730, Peoples R China
来源：
ACS APPLIED MATERIALS & INTERFACES | 2019年 / 11卷 / 14期
基金：
中国国家自然科学基金;
关键词：
near-infrared (NIR) luminogens; aggregation-induced emission (AIE); mitochondria imaging; pH-sensitive probe; LIVING CELLS; PROBE; FLUORESCENCE;
D O I：
10.1021/acsami.9b02228
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Mitochondria are crucial in the process of oxidative metabolism and apoptosis. Their morphology is greatly associated with the development of certain diseases. For specific and long-term imaging of mitochondrial morphology, we synthesized a new mitochondria-targeted near-infrared (NIR) fluorescent probe (TPE-Xan-In) by incorporating TPE with a NIR merocyanine skeleton (Xan-In). TPE-Xan-In displayed both absorption (660 nm) and emission peaks (743 nm) in the NIR region. Moreover, it showed aggregation-induced emission properties at neutral pH and specifically illuminated mitochondria with good biocompatibility, superior photostability, and high tolerance to mitochondrial membrane potential changes. With a pH-responsive unit, hydroxyl xanthene (Xan), the probe exhibited a pH-sensitive fluorescence emission in the range of pH 4.0-7.0, which indicated its potential in long-term tracking of pH and morphology changes of mitochondria in the biomedical research studies.
引用
收藏
页码：13134 / 13139
页数：6
相关论文
共 20 条
  • [1] An AIE-active hemicyanine fluorogen with stimuli-responsive red/blue emission: extending the pH sensing range by "switch+knob" effect
    Chen, Sijie
    Liu, Jianzhao
    Liu, Yang
    Su, Huimin
    Hong, Yuning
    Jim, Cathy K. W.
    Kwok, Ryan T. K.
    Zhao, Na
    Qin, Wei
    Lam, Jacky W. Y.
    Wong, Kam Sing
    Tang, Ben Zhong
    [J]. CHEMICAL SCIENCE, 2012, 3 (06) : 1804 - 1809
  • [2] DAVIS S, 1985, J BIOL CHEM, V260, P3844
  • [3] Tumors as Organs: Complex Tissues that Interface with the Entire Organism
    Egeblad, Mikala
    Nakasone, Elizabeth S.
    Werb, Zena
    [J]. DEVELOPMENTAL CELL, 2010, 18 (06) : 884 - 901
  • [4] Far Red/Near-Infrared AIE Dots for Image-Guided Photodynamic Cancer Cell Ablation
    Feng, Guangxue
    Wu, Wenbo
    Xu, Shidang
    Liu, Bin
    [J]. ACS APPLIED MATERIALS & INTERFACES, 2016, 8 (33) : 21193 - 21200
  • [5] Mitochondria-Targeted Cancer Therapy Using a Light-Up Probe with Aggregation-Induced-Emission Characteristics
    Hu, Qinglian
    Gao, Meng
    Feng, Guangxue
    Liu, Bin
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2014, 53 (51) : 14225 - 14229
  • [6] MONITORING OF RELATIVE MITOCHONDRIAL-MEMBRANE POTENTIAL IN LIVING CELLS BY FLUORESCENCE MICROSCOPY
    JOHNSON, LV
    WALSH, ML
    BOCKUS, BJ
    CHEN, LB
    [J]. JOURNAL OF CELL BIOLOGY, 1981, 88 (03) : 526 - 535
  • [7] Mitochondrial quality control and communications with the nucleus are important in maintaining mitochondrial function and cell health
    Kotiadis, Vassilios N.
    Duchen, Michael R.
    Osellame, Laura D.
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2014, 1840 (04): : 1254 - 1265
  • [8] Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe
    Leevy, W. Matthew
    Gammon, Seth T.
    Jiang, Hua
    Johnson, James R.
    Maxwell, Dustin J.
    Jackson, Erin N.
    Marquez, Manuel
    Piwnica-Worms, David
    Smith, Bradley D.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (51) : 16476 - 16477
  • [9] A Photostable AIE Luminogen for Specific Mitochondrial Imaging and Tracking
    Leung, Chris Wai Tung
    Hong, Yuning
    Chen, Sijie
    Zhao, Engui
    Lam, Jacky Wing Yip
    Tang, Ben Zhong
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (01) : 62 - 65
  • [10] Aggregation-Induced Fluorescence Probe for Monitoring Membrane Potential Changes in Mitochondria
    Li, Jun
    Kwon, Nahyun
    Jeong, Yerin
    Lee, Songyi
    Kim, Gyoungmi
    Yoon, Juyoung
    [J]. ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (15) : 12150 - 12154
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