Comparative evaluation of cytotoxicity and phototoxicity of mono and diacylglycerol amino acid-based surfactants

被引:20
作者
Pilar Vinardell, Maria [1 ,5 ]
Benavides, Tomas [1 ]
Mitjans, Montserrat [1 ]
Rosa Infante, Maria [3 ]
Clapes, Pere [4 ]
Clothier, Richard [2 ]
机构
[1] Univ Barcelona BCN, Fac Farm, Dept Fisiol, Div 4, Barcelona 08028, Spain
[2] Univ Nottingham, QMC, Sch Biomed Sci, FRAME Alternat Lab, Nottingham NG7 2RD, England
[3] IIAQ CSIC BCN, Dept Surfactant Technol, Barcelona, Spain
[4] IIAQ CSIC BCN, Dept Peptide & Prot Chem, Barcelona, Spain
[5] CSIC 8004 BCN, Unidad Asociada, Barcelona, Spain
关键词
Surfactants; Cytotoxicity; Phototoxicity; 3T3; HaCaT;
D O I
10.1016/j.fct.2008.10.007
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Extensive efforts have been made, recently, to find surfactants with lower irritancy potential than those presently commercially employed in pharmaceutical and cosmetic preparations. Cytotoxic and phototoxic effects of novel mono and diacylglycerol amino acid-based surfactants (glutamic acid, or arginine) were evaluated. All tested surfactants showed a clear concentration-response relationship to two immortalized cell lines, routine fibroblast cell line, 3T3, and one human keratinocyte cell line, HaCaT, demonstrated by and decrease of NR uptake. Concentrations resulting in 50% inhibition of NR uptake (IC50) range from 30 to 300 mu g mL(-1). The potential phototoxicity which could result in irritant products, was determined by modulated cytotoxicity via the resazurin reduction to resorufin and neutral red uptake (NRU) endpoints. Surfactants with two chains showed, in general, less cytotoxic but higher phototoxic effect than surfactants with only one chain. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3837 / 3841
页数:5
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