Discrimination of Isobaric Leu/Ile Residues by MALDI In-Source Decay Mass Spectrometry

被引：32

作者：

Asakawa, Daiki ^{[1
,2
,3
]}

Smargiasso, Nicolas ^{[1
,2
]}

De Pauw, Edwin ^{[1
,2
]}

机构：

[1] Univ Liege, Dept Chem, Mass Spectrometry Lab, Liege, Belgium

[2] Univ Liege, GIGA R, Mass Spectrometry Lab, Liege, Belgium

[3] Yokohama City Univ, Grad Sch Nano Biosci, Mass Spectrometry Lab, Yokohama, Kanagawa 232, Japan

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2013年 / 24卷 / 02期

基金：

日本学术振兴会;

关键词：

Initial velocity; Side-chain loss; 1,5-Diaminonapthalene; Negative-ion mode; Top-down sequencing; ELECTRON-TRANSFER DISSOCIATION; LASER-DESORPTION IONIZATION; FRAGMENTATION PROCESSES; GAS-PHASE; PEPTIDES; MATRIX; IONS; PROTEIN; TIME; ISOLEUCINE;

D O I：

10.1007/s13361-012-0523-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MALDI in-source decay (ISD) has been used for top-down sequencing of proteins. The use of the matrix 1,5-diaminonapthalene (1,5-DAN) gave abundant w ions, which are formed from the unimolecular dissociation of zaEuro cent radical fragments via alpha cleavage reaction and thus help identify which of the isobaric amino acids, Leu or Ile, is present. The high abundance of w ions in MALDI-ISD with 1,5-DAN results from the low collision rate in the MALDI plume. MALDI-ISD with 1,5-DAN appears to be an useful method for the top-down sequencing of proteins, including discrimination of Leu and Ile near the C-terminal end.

引用

页码：297 / 300

页数：4

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