Decellularized Nucleus Pulposus Matrix/Chitosan Hybrid Hydrogels for Nucleus Pulposus Tissue Engineering

被引：4

作者：

Zhang, Yu ^{[1
,2
,3
,4
]}

Li, Yifeng ^{[3
,4
]}

Liu, Chen ^{[3
,4
]}

Shang, Xifu ^{[1
,2
]}

机构：

[1] Shandong Univ, Cheeloo Coll Med, Jinan 250012, Shandong, Peoples R China

[2] Univ Sci & Technol China, Affiliated Hosp USTC 1, Div Life Sci & Med, Hefei, Peoples R China

[3] Key Lab Noncoding RNA Transformat Res Anhui Highe, Wuhu, Peoples R China

[4] Wannan Med Coll, Dept Spine Surg, Yijishan Hosp, Wuhu 241001, Anhui, Peoples R China

来源：

GLOBAL SPINE JOURNAL | 2024年 / 14卷 / 04期

基金：

中国国家自然科学基金;

关键词：

nucleus pulposus stem cells; decellularized nucleus pulposus matrix; chitosan; genipin; gene expression; INTERVERTEBRAL DISC; CELLS;

D O I：

10.1177/21925682221135768

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Study Design: Basic research Objective: To prepared 3 DNPM/chitosan hybrid hydrogels and chose the best DNPM/chitosan hybrid hydrogel for NP tissue engineering. Methods: Three DNPM/chitosan hybrid hydrogels were fabricated by changing the ratio of the decellularized NP matrix to chitosan and crosslinking with genipin. NP stem cells (NPSCs) were cultured on the hybrid hydrogels and their proliferation, morphology, and gene expression were evaluated. Finally, an in vivo experiment was performed to evaluate the immune response to the hydrogels. Results: The adhered NPSCs proliferated well on the hybrid hydrogel. The gene expression of NP-related collagen type II, aggrecan, and Sox-9 from NPSCs cultured on DNPM/chitosan hybrid hydrogel-I was greater than from cells cultured on DNPM/chitosan hybrid hydrogel-2 and DNPM/chitosan hybrid hydrogel-3. Few inflammatory cells were observed during the in vivo experiment with DNPM/chitosan hybrid hydrogel-I. Conclusions: DNPM/chitosan hybrid hydrogel-1 is a potential candidate scaffold for NP tissue engineering.

引用

页码：1175 / 1183

页数：9

共 26 条

[1] Intervertebral disc arthroplasty [J].