Microfluidic aqueous two phase system for leukocyte concentration from whole blood

被引：101
|
作者：
SooHoo, Jeffrey R. [1 ]
Walker, Glenn M.
机构：
[1] N Carolina State Univ, Joint Dept Biomed Engn, Raleigh, NC 27695 USA
来源：
BIOMEDICAL MICRODEVICES | 2009年 / 11卷 / 02期
关键词：
Microfluidics; Leukocyte; Aqueous two phase separation; Blood; PEG; Dex; MEMBRANE-SURFACE PROPERTIES; PHASE SYSTEMS; COUNTERCURRENT DISTRIBUTION; FLOW FRACTIONATION; CELLS; PARTITION; LYMPHOCYTES; SEPARATION; POLYMER; DEVICE;
D O I：
10.1007/s10544-008-9238-8
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Leukocytes from a whole blood sample were concentrated using a microfluidic aqueous two phase system (mu ATPS). Whole blood was simultaneously exposed to polyethylene glycol (PEG) and dextran (Dex) phase streams and cells were partitioned based on their differential affinity for the streams. The laminar flow characteristic of microfluidic devices was used to create zero, one, and two stable interfaces between the polymer streams. Three different patterns of three polymer streams each were evaluated for their effectiveness in concentrating leukocytes: immiscible PEG-PEG-Dex, immiscible Dex-PEG-Dex, and miscible PEG-PBS-Dex. The most effective configuration was the Dex-PEG-Dex stream pattern which on average increased the ratio of leukocytes to erythrocytes by a factor of 9.13 over unconcentrated blood.
引用
收藏
页码：323 / 329
页数：7
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