MALAT-1 interacts with hnRNP C in cell cycle regulation

As a conserved non-coding RNA gene, transcripts of MALAT-1 localize predominately in the nucleus. However in G2/M cell cycle phase, MALAT-1 transcripts were surprisingly found to translocate from the nucleus into the cytoplasm. Investigation also found that in this process MALAT-1 interacts with an abundant nuclear factor, hnRNP C protein. Using a loss-of-function assay, we found that downregulation of MALAT-1 expression compromised the cytoplasmic translocation of hnRNP C in the G2/M phase and resulted in G2/M arrest. In addition to characterize the physiological interaction between MALAT-1 and hnRNP C, our study also highlights the role of MALAT-1 in cell cycle regulation. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.