Molecular combing and its application in clinical settings

被引：0
作者：
Wang, Yiping [1 ,2 ]
Kumar, Kishore Ramesh [1 ]
Liehr, Thomas [1 ]
机构：
[1] Friedrich Schiller Univ, Jena Univ Hosp, Inst Human Genet, Klinikum 1, D-07747 Jena, Germany
[2] Natl Engn Ctr Prot Drugs, Jinze Grp, Beijing Jinze Med Lab, Bldg A,Bldg 1,33 Sci Pk Rd,1F, Beijing, Peoples R China
来源：
MOLECULAR CYTOGENETICS | 2022年 / 15卷 / 01期
关键词：
Molecular combing; Fluorescence in situ hybridization (FISH); Genomics; Constitutional genetic diseases; Acquired genetic diseases; IN-SITU HYBRIDIZATION; HUMAN-CHROMOSOMES; FISH; DNA; REARRANGEMENTS; GENOME; GENES; DISEASE; BREAST; BRCA1;
D O I：
10.1186/s13039-022-00628-8
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Molecular combing technology (MCT) is an effective means for stretching DNA molecules and making them thus accessible for in situ studies. MCT uses the force exerted in the process of liquid flow via surface tension to stretch DNA molecules and spread them on solid surfaces, i.e. glass cover slips. Many DNA molecules can be stretched at the same time in parallel and neatly arranged side-by-side, making the approach convenient for statistical analysis. Accordingly, DNA replication and transcription can be studied at the single molecule level. In this paper, the principle, experimental methods, important applications, advantages and shortcuts of MCT in medical field are presented and discussed.
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页数：7
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