Physiological effects of sustained blockade of excitatory synaptic transmission on spontaneously active developing neuronal networks - an inquiry into the reciprocal linkage between intrinsic biorhythms and neuroplasticity in early ontogeny

被引:105
作者
Corner, MA
van Pelt, J
Wolters, PS
Baker, RE
Nuytinck, RH
机构
[1] Univ Amsterdam, Acad Med Ctr, Netherlands Inst Brain Res, NL-1105 AZ Amsterdam, Netherlands
[2] Univ Amsterdam, Neurosci Res Sch, Amsterdam, Netherlands
关键词
explant cultures; visual cortex; spontaneous bioelectric activity; spike-train analysis; burst firing; NMDA receptors; AMPA receptors; GABAergic inhibition; minute-order rhythms; neural network formation;
D O I
10.1016/S0149-7634(01)00062-8
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
Spontaneous bioelectric activity (SBA) taking the form of extracellularly recorded spike trains (SBA) has been quantitatively analyzed in organotypic neonatal rat visual cortex explants at different ages in vitro, and the effects investigated of both short- and long-term pharmacological suppression of glutamatergic synaptic transmission. In the presence of APV, a selective NMDA receptor blocker, 1-2- (but not 3-) week-old cultures recovered their previous SBA levels in a matter of hours, although in imitation of the acute effect of the GABAergic inhibitor picrotoxin (PTX), bursts of action potentials were abnormally short and intense. Cultures treated either overnight or chronically for 1-3 weeks with APV, the AMPA/kainate receptor blocker DNQX, or a combination of the two were found to display very different abnormalities in their firing patterns. NMDA receptor blockade for 3 weeks produced the most severe deviations from control SBA, consisting of greatly prolonged and intensified burst firing with a strong tendency to be broken up into trains of shorter spike clusters. This pattern was most closely approximated by acute GABAergic disinhibition in cultures of the same age, but this latter treatment also differed in several respects from the chronic-APV effect. In 2-week-old explants, in contrast, it was the APV + DNQX treated group which showed the most exaggerated spike bursts. Functional maturation of neocortical networks, therefore, may specifically require NMDA receptor activation (not merely a high level of neuronal firing) which initially is driven by endogenous rather than afferent evoked bioelectric activity. Putative cellular mechanisms are discussed in the context of a thorough review of the extensive but scattered literature relating activity-dependent brain development to spontaneous neuronal firing patterns. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:127 / 185
页数:59
相关论文
共 833 条
[1]   Synaptic plasticity: taming the beast [J].
Abbott, L. F. ;
Nelson, Sacha B. .
NATURE NEUROSCIENCE, 2000, 3 (11) :1178-1183
[2]  
ABBOTT LF, 2002, IN PRESS MODELING NE
[3]  
Abeles M., 1982, LOCAL CORTICAL CIRCU, DOI DOI 10.1007/978-3-642-81708-3
[4]   Metaplasticity: A new vista across the field of synaptic plasticity [J].
Abraham, WC ;
Tate, WP .
PROGRESS IN NEUROBIOLOGY, 1997, 52 (04) :303-323
[5]   Metaplasticity: The plasticity of synaptic plasticity [J].
Abraham, WC ;
Bear, MF .
TRENDS IN NEUROSCIENCES, 1996, 19 (04) :126-130
[6]   DEVELOPMENT OF UNIT-ACTIVITY IN LATERAL GENICULATE-NUCLEUS OF KITTEN [J].
ADRIEN, J ;
ROFFWARG, HP .
EXPERIMENTAL NEUROLOGY, 1974, 43 (01) :261-275
[7]   ONTOGENY OF UNIT-ACTIVITY IN THE RAPHE DORSALIS OF THE BEHAVING KITTEN - ITS RELATIONSHIP WITH THE STATES OF VIGILANCE [J].
ADRIEN, J ;
LANFUMEY, L .
BRAIN RESEARCH, 1986, 366 (1-2) :10-21
[8]  
AGHAJANIAN G. K., 1967, BRAIN RES, V6, P716, DOI 10.1016/0006-8993(67)90128-X
[9]   REPETITIVE BURST-FIRING NEURONS IN THE DEEP LAYERS OF MOUSE SOMATOSENSORY CORTEX [J].
AGMON, A ;
CONNORS, BW .
NEUROSCIENCE LETTERS, 1989, 99 (1-2) :137-141
[10]   NMDA RECEPTOR-MEDIATED CURRENTS ARE PROMINENT IN THE THALAMOCORTICAL SYNAPTIC RESPONSE BEFORE MATURATION OF INHIBITION [J].
AGMON, A ;
ODOWD, DK .
JOURNAL OF NEUROPHYSIOLOGY, 1992, 68 (01) :345-349