Probing gender-specific metabolism differences in humans by nuclear magnetic resonance-based metabonomics

被引:204
作者
Kochhar, S [1 ]
Jacobs, DM [1 ]
Ramadan, Z [1 ]
Berruex, F [1 ]
Fuerhoz, A [1 ]
Fay, LB [1 ]
机构
[1] Nestle Res Ctr, BioAnalyt Sci Dept, CH-1000 Lausanne 26, Switzerland
关键词
metabonomics; chemometrics; NMR spectroscopy; gender variation; citric acid cycle;
D O I
10.1016/j.ab.2006.02.033
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The measurement of metabolite profiles that are interpreted to yield biomarkers using multivariate data analysis is now a well-established approach for gaining an improved understanding of the impact of genetic modifications, toxicological and therapeutic interventions.. and exposure to stimuli (e.g., noxious agents, stressors, nutrients) on the network of transcripts, proteins, and metabolites present in cells, tissues, or whole organisms. This has been termed metabonomics. In this study, multivariate analysis of H-1 nuclear magnetic resonance (NMR) spectra of metabolite profiles of urine and plasma from 150 healthy humans revealed that in young people and/or individuals with low body mass indexes, females had higher rates of lipid biosynthesis than did males, whereas males had higher rates of protein turnover than did females. With increasing age, overall lipid biosynthesis decreased in females, whereas metabolism increasingly favored lipid synthesis over protein turnover in males. By relating the derived metabonomic data to known metabolic pathways and published biochemical data, it appears that females synthesize relatively more lipoproteins and unsaturated lipids than do males. Furthermore, the changes in lipid biosynthesis and urinary citrate excretion in females showed a positive correlation. Estrogen most likely plays an essential role in the regulation of, and communication between, protein and lipid biosynthesis by controlling pH in mitochondria and the cytoplasm and hence the observed altered citrate levels. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:274 / 281
页数:8
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