Online photolytic optical gating of caged fluorophores in capillary zone electrophoresis utilizing an ultraviolet light-emitting diode

被引：3

作者：

Gallagher, Elyssia S. ^{[1
]}

Comi, Troy J. ^{[1
]}

Braun, Kevin L. ^{[2
]}

Aspinwall, Craig A. ^{[1
,3
]}

机构：

[1] Univ Arizona, Dept Chem & Biochem, Tucson, AZ USA

[2] Beloit Coll, Dept Chem, Beloit, WI 53511 USA

[3] Univ Arizona, Inst Bio5, Tucson, AZ USA

来源：

ELECTROPHORESIS | 2012年 / 33卷 / 18期

关键词：

Capillary zone electrophoresis; Hadamard transform; Photolytic optical gating; Ultraviolet light-emitting diode; INDUCED FLUORESCENCE DETECTION; COMPREHENSIVE 2-DIMENSIONAL SYSTEM; FOURIER-TRANSFORM DETECTION; HADAMARD-TRANSFORM; LIQUID-CHROMATOGRAPHY; INJECTION VOLUME; MICROCOLUMN-LC; NERVOUS-SYSTEM; SEGMENTED FLOW; NITRIC-OXIDE;

D O I：

10.1002/elps.201200279

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Photolytic optical gating (POG) facilitates rapid, on-line and highly sensitive analyses, though POG utilizes UV lasers for sample injection. We present a low-cost, more portable alternative, employing an ultraviolet light-emitting diode (UV-LED) array to inject caged fluorescent dyes via photolysis. Utilizing the UV-LED array, labeled amino acids were injected with nanomolar limits of detection (270 +/- 30 nM and 250 +/- 30 nM for arginine and citrulline, respectively). When normalized for the difference in light intensity, the UV-LED array provides comparable sensitivity to POG utilizing UV lasers. Additionally, the UV-LED array yielded sufficient beam quality and stability to facilitate coupling with a Hadamard transform, resulting in increased sensitivity. This work shows, for the first time, the use of an UV-LED for online POG with comparable sensitivity to conventional laser sources but at a lower cost.

引用

页码：2903 / 2910

页数：8