Structural changes of methemoglobin after adsorption on bioactive glass, as a function of surface functionalization and salt concentration

被引：17

作者：

Gruian, C. ^{[1
,2
,3
]}

Vulpoi, A. ^{[1
,2
]}

Steinhoff, H. -J. ^{[3
]}

Simon, S. ^{[1
,2
]}

机构：

[1] Univ Babes Bolyai, Fac Phys, Cluj Napoca 400084, Romania

[2] Univ Babes Bolyai, Interdisciplinary Res Inst BioNanoSci, Cluj Napoca 400084, Romania

[3] Univ Osnabruck, Dept Phys, D-49069 Osnabruck, Germany

来源：

JOURNAL OF MOLECULAR STRUCTURE | 2012年 / 1015卷

关键词：

Electron paramagnetic resonance; Protein adsorption; Bioactive glasses; Salt concentration; SECONDARY STRUCTURE; PROTEIN ADSORPTION; T4; LYSOZYME; HEMOGLOBIN; BINDING; SITE; GLUTARALDEHYDE; ALBUMIN; TIRF; EPR;

D O I：

10.1016/j.molstruc.2012.01.045

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Functional protein adsorption at liquid solid interfaces has been intensively studied in the last years, however it is difficult to evidence directly conformational changes of the protein which are likely to appear upon adsorption. Spin labeling in combination with Electron Paramagnetic Resonance (EPR) spectroscopy was applied in this study to investigate adsorption of horse methemoglobin to bioactive glass (BC) similar in composition with 4555 Bioglass (R). X-band cw-EPR spectra of spin labeled methemoglobin in solution were compared to those obtained after adsorption on bioactive glass surface (functionalized and non-functionalized with glutaraldehyde), to extract information of the structure and dynamics in the vicinity of position beta-93. The concentration of methemoglobin adsorbed on BC substrate was determined from the intensity of cw-EPR spectra and correlated with images obtained by Scanning Electron Microscopy (SEM). Line shape analysis of the EPR spectra revealed that ionic strength does not induce significant conformational changes in the protein structure upon adsorption, however, the chemical treatment applied to the bioactive glass surface positively influences protein adsorption. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：20 / 26

页数：7

共 44 条

[21]

MACDONALD SL, 1994, METHOD ENZYMOL, V231, P287

[22] EFFECT OF SLOW ROTATIONAL MOTION ON PARAMAGNETIC-RESONANCE SPECTRA [J].

MCCALLEY, RC ;

MCCONNELL, HM ;

SHIMSHICK, EJ .

CHEMICAL PHYSICS LETTERS, 1972, 13 (02) :115-+

[23]

McConnell H M, 1970, Q Rev Biophys, V3, P91

[24] SPIN-LABELED HEMOGLOBIN DERIVATIVES IN SOLUTION POLYCRYSTALLINE SUSPENSIONS AND SINGLE CRYSTALS [J].

MCCONNELL, HM ;

DEAL, W ;

OGATA, RT .

BIOCHEMISTRY, 1969, 8 (06) :2580-+

[25]

McGuire J., 1995, PROTEINS INTERFACES

[26] SPIN-LABELLED HAEMOGLOBINS - A STRUCTURAL INTERPRETATION OF ELECTRON PARAMAGNETIC RESONANCE SPECTRA BASED ON X-RAY ANALYSIS [J].

MOFFAT, JK .

JOURNAL OF MOLECULAR BIOLOGY, 1971, 55 (02) :135-&

[27]

Nanci A, 1998, J BIOMED MATER RES, V40, P324

[28] SPIN-LABEL STUDY OF HEMOGLOBIN CONFORMATIONS IN SOLUTION [J].

OGAWA, S ;

MCCONNELL, HM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 58 (01) :19-+

[29] REACTION OF GLUTARALDEHYDE WITH AMINO AND THIOL COMPOUNDS [J].

OKUDA, K ;

URABE, I ;

YAMADA, Y ;

OKADA, H .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1991, 71 (02) :100-105

[30] Structure and dynamics of annexin 12 bound to a planar lipid bilayer [J].

Risse, T ;

Hubbell, WL ;

Isas, JM ;

Haigler, HT .

PHYSICAL REVIEW LETTERS, 2003, 91 (18) :188101-188101

← 1 2 3 4 5 →