Automatic three-dimensional localization of micro-particles using digital holographic microscopy

被引：0

作者：

Antkowiak, Maciej ^{[1
]}

Callens, Natacha ^{[1
]}

Yourassowsky, Catherine ^{[1
]}

Dubois, Frank ^{[1
]}

机构：

[1] Univ Libre Bruxelles, Micrograv Res Ctr, B-1050 Brussels, Belgium

来源：

Interferometry XIV: Applications | 2008年 / 7064卷

关键词：

Digital Holography; Microscopy; Auto-focusing; Object Detection;

D O I：

10.1117/12.799852

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We present a numerical technique for automatic extended focused imaging and three-dimensional analysis of microparticle field observed in a digital holographic microscope working in transmission. We use Fourier method for the extraction of complex amplitude from the single exposition digital holograms. We create a synthetic extended focused image (EFI) using the focus plane determination method based on the integrated amplitude modulus. We apply the refocusing criterion locally for each pixel, using small overlapping windows, in order to obtain a depth map and a synthetic image in which all objects are refocused independent from their refocusing distance. The obtained synthetic EFI allows us to perform image segmentation and object detection. We improve the accuracy of vertical localization using an additional refining procedure in which each particle is treated separately. A successful application of this technique in the analysis of microgravity particle flow experiment is presented.

引用

页数：12

共 26 条

[1] Application of wavelet transform to hologram analysis:: three-dimensional location of particles [J].

Buraga-Lefebvre, C ;

Coëtmellec, S ;

Lebrun, D ;

Özkul, C .

OPTICS AND LASERS IN ENGINEERING, 2000, 33 (06) :409-421

[2]

CALLENS N, 2005, THESIS U PARIS 6 FRA

[3] Digital holography for quantitative phase-contrast imaging [J].

Cuche, E ;

Bevilacqua, F ;

Depeursinge, C .

OPTICS LETTERS, 1999, 24 (05) :291-293

[4] Digital holographic microscopy with reduced spatial coherence for three-dimensional particle flow analysis [J].

Dubois, F ;

Callens, N ;

Yourassowsky, C ;

Hoyos, M ;

Kurowski, P ;

Monnom, O .

APPLIED OPTICS, 2006, 45 (05) :864-871

[5] Partial spatial coherence effects in digital holographic microscopy with a laser source [J].

Dubois, F ;

Requena, MLN ;

Minetti, C ;

Monnom, O ;

Istasse, E .

APPLIED OPTICS, 2004, 43 (05) :1131-1139

[6] Focus plane detection criteria in digital holography microscopy by amplitude analysis [J].

Dubois, Frank ;

Schockaert, Cedric ;

Callens, Natacha ;

Yourassowsky, Catherine .

OPTICS EXPRESS, 2006, 14 (13) :5895-5908

[7] Extended focused image in microscopy by digital holography [J].

Ferraro, P ;

Grilli, S ;

Alfieri, D ;

De Nicola, S ;

Finizio, A ;

Pierattini, G ;

Javidi, B ;

Coppola, G ;

Striano, V .

OPTICS EXPRESS, 2005, 13 (18) :6738-6749

[8] A SYSTEM BASED ON SPLIT-FLOW LATERAL-TRANSPORT THIN (SPLITT) SEPARATION CELLS FOR RAPID AND CONTINUOUS PARTICLE FRACTIONATION [J].

GIDDINGS, JC .

SEPARATION SCIENCE AND TECHNOLOGY, 1985, 20 (9-10) :749-768

[9] THE USE OF SELF-ENTROPY AS A FOCUS MEASURE IN DIGITAL HOLOGRAPHY [J].

GILLESPIE, J ;

KING, RA .

PATTERN RECOGNITION LETTERS, 1989, 9 (01) :19-25

[10] Real-time automated 3D sensing, detection, and recognition of dynamic biological micro-organic events [J].

Javidi, B ;

Yeom, S ;

Moon, I ;

Daneshpanah, M .

OPTICS EXPRESS, 2006, 14 (09) :3806-3829

← 1 2 3 →