Protons and single nucleotide polymorphism detection: A simple use for the ion sensitive field effect transistor

被引：64
作者：
Purushothaman, S [1 ]
Toumazou, C
Ou, CP
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, London SW7 2AZ, England
[2] DNA Elect, London WC1N 2EB, England
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2006年 / 114卷 / 02期
关键词：
DNA; DNA sequencing; genetic diagnosis; testing; ISFET; pH; real time sequencing;
D O I：
10.1016/j.snb.2005.06.069
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The identification of single nucleotide polymorphisms (SNP) is a subject receiving considerable attention owing to the possibility of diagnosing and screening for diseases they are connected with. Currently, the emphasis for their detection is using DNA hybridisation methods and the development of novel, mainly fluorescent based methods for hybridisation detection. We report on a new method for identifying SNPs using Ion Sensitive Field Effect Transistor technology. Through the detection of single nucleotide mismatches, we show a procedure for effectively sequencing one base and therefore a mechanism for sequencing new DNA. (c) 2005 Published by Elsevier B.V.
引用
收藏
页码：964 / 968
页数：5
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