Induction by ozone of ethylene production and an ACC oxidase cDNA in rice (Oryza sativa L.) leaves

被引:4
|
作者
Ohki, T
Matsui, H
Nagasaka, A
Yoshioka, T
Watanabe, A
Satoh, S
机构
[1] Tohoku Univ, Grad Sch Agr Sci, Lab Bioadapt, Aoba Ku, Sendai, Miyagi 9818555, Japan
[2] Tohoku Univ, Grad Sch Agr Sci, Biophys Chem Lab, Aoba Ku, Sendai, Miyagi 9818555, Japan
关键词
1-aminocyclopropane-1-carboxylate (ACC) oxidase; ethylene; Oryza sativa; ozone; rice;
D O I
10.1023/A:1006229421785
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Exposure to ozone at 1 mu l l(-1) for 6 h induced ethylene production in rice (Oryza sativa L. cv. Hitomebore) leaves. The stimulation of ethylene production was detectable 2 h after the start of the exposure to ozone, and lasted for 6 h after the exposure. A 429-bp cDNA fragment encoding ACC oxidase was obtained by RT-PCR from ozone-treated rice leaves. Its nucleotide sequence and deduced amino-acid sequence had 97.2% and 94.4% identity, respectively, to those of OS1A1COX, which was previously obtained from deepwater rice. The abundance of the cDNA increased in accordance with the induction of ethylene production by the exposure to ozone.
引用
收藏
页码:123 / 127
页数:5
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