Hypoxia-increased RAGE expression regulates chemotaxis and pro inflammatory cytokines release through nuclear translocation of NF-κ B and HIFI α in THP-1 cells

被引:18
作者
Bai, Wei [1 ]
Zhou, Jing [1 ]
Zhou, Na [2 ]
Liu, Qin [1 ]
Cui, Jian [1 ]
Zou, Wei [3 ]
Zhang, Wei [1 ]
机构
[1] Nanchang Univ, Affiliated Hosp 1, Dept Resp Med, 17 Yongwaizheng Ave, Nanchang 330006, Jiangxi, Peoples R China
[2] Nanchang Third Hosp, Dept Resp Med, Nanchang, Jiangxi, Peoples R China
[3] Hunan Prov Maternal & Child Hlth Hosp, Key Lab Hunan Prov Tradit Chinese Med Obstet & Gy, 53 Xiangchun Rd, Changsha 410008, Hunan, Peoples R China
基金
中国国家自然科学基金;
关键词
Hypoxia; The receptor for advanced glycation end products (RAGE); NF-kappa B; HIF-1; alpha; Chemotaxis; Inflammation; END-PRODUCTS RAGE; ENDOTHELIAL-CELLS; OXIDATIVE STRESS; EPITHELIAL-CELLS; RECEPTOR; ACTIVATION; GENE;
D O I
10.1016/j.bbrc.2017.12.084
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The potential role of hypoxia in mediating the receptor for advanced glycation end products (RAGE) expression deserves to be confirmed. And the role of RAGE in hypoxia-induced chemotaxis and inflammation is still unclear. In present study, THP-1 cells were pretreated with siRNA to block HIFI cc,NF-kappa B, or RAGE, followed by exposed to hypoxia (combined with H2O2 or SNP), and then RAGE expression, nuclear translocation of HIFI cc and NF-kappa B, release of TNF-alpha and IL-1 beta, as well as expression of MCP-1 and CCR2 were measured. The results revealed that RAGE mRNA and protein in THP-1 cells were significantly increased after exposed into hypoxia atmosphere, especially into the solution containing SNP or H2O2. Moreover, SNP or H2O2 exposure could further amplify hypoxia-induced nuclear translocation of HIF-1 alpha and NF-kappa B. Knockdown HIF-1 alpha or NF-kappa B by siRNAs could reduce hypoxia-and oxidative stress-induced RAGE hyper-expression. And pretreatment THP-1 cells with RAGE siRNA or NF-kappa B siRNA could reduce hypoxia- and oxidative stress-induced expression of MCP-I and CCR2, and release of TNF-alpha and IL-1 beta. Thus, hypoxia not only increases RAGE expression in THP-1 cells by promoting nuclear translocation of NF-kappa B and HIFI alpha, but also regulates chemotaxis and pro-inflammatory cytokines release, which may be partially mediated through upregulation of RAGE expression. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码:2282 / 2288
页数:7
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