Cloning and characterization of a glycosyltransferase gene involved in the biosynthesis of anthracycline antibiotic β-rhodomycin from Streptomyces violaceus

被引：11

作者：

Miyamoto, Y

Johdo, O

Nagamatsu, Y

Yoshimoto, A

机构：

[1] Hiroshima Univ, Fac Appl Biol Sci, Dept Appl Biochem, Higashihiroshima 7398528, Japan

[2] Mercian Corp, Cent Res Labs, Fujisawa, Kanagawa 2510057, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 206卷 / 02期

关键词：

glycosyltransferase; beta-rhodomycin; biosynthesis; anthracycline; Streptomyces gene;

D O I：

10.1111/j.1574-6968.2002.tb11003.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A glycosyltransferase gene, rhoG, involved in the biosynthesis of the anthracycline antibiotic beta-rhodomycin was isolated as a 4.1-kb DNA fragment containing rhoG and its flanking region from Streptomyces violaceus by degenerate and inverse PCR. Sequencing analysis showed that rhoG was located in a gene cluster involved in the biosynthesis of the constitutive deoxysugar of beta-rhodomycin. The function of rhoG was verified by gene disruption, which was generated by replacing the internal 0.9-kb region of S. violaceus chromosome with a fragment including the SacI-blunted region. The rhoG disruption resulted in complete loss of beta-rhodomycin productivity. along with the accumulation of a non-glycosyl intermediate e-rhodomycinone. In addition, the complementation test demonstrated that rhoG restored beta-rhodomycin production in this gene disruptant. These results indicated that rhoG is the glycosyltransferase gene responsible for the glycosylation of epsilon-rhodomycinone in beta-rhodomycin biosynthesis. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：163 / 168

页数：6

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