In vitro maturation of neonatal porcine islets -: A novel model for the study of islet development and xenotransplantation

被引：0

作者：

Binette, TM ^{[1
]}

Dufour, JM ^{[1
]}

Korbutt, GS ^{[1
]}

机构：

[1] Univ Alberta, Dept Surg, Surg Med Res Inst, Edmonton, AB T6G 2N8, Canada

来源：

BIOARTIFICIAL ORGANS III: TISSUE SOURCING, IMMUNOISOLATION, AND CLINICAL TRIALS | 2001年 / 944卷

关键词：

maturation; neonatal porcine islets; islet development; PDX-1; xenotransplantation;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The mechanisms involved in islet neogenesis have remained largely unexplored due to lack of an appropriate model. Furthermore, with the recent advances in islet transplantation, the need for alternative islet tissue sources is greater than ever. Therefore, the authors have refined a neonatal porcine islet (NPI) maturation model that offers an ideal tool to gain insight into islet growth as well as an alternative source of transplantable tissue. Recent knowledge in islet growth has resulted in endocrine tissue being derived from human pancreatic precursor tissue in vitro. The potential for large scale production of endocrine tissue in vitro has been indicated, however, more investigation must be done on the various signals and pathways involved in pancreatic development to optimize this technique. The authors believe that their NPI in vitro maturation model provides an ideal tool to study islet growth and maturation. Transduction of the NPI to overexpress genes of interest (i.e., PDX-1) or exposure of the NPI to various culture conditions will allow us to determine the effects on islet maturation. An understanding of NPI development gained will not only allow us to mature this unlimited tissue source for optimal xenotransplantation, but also elude to how human pancreatic endocrine precursor cells may be used to solve the current islet tissue supply problem.

引用

页码：47 / 61

页数：15

共 38 条

[11] Enhancement of In Vitro and In Vivo Function of Agarose-Encapsulated Porcine Islets by Changes in the Islet Microenvironment [J].

Holdcraft, Robert W. ;

Gazda, Lawrence S. ;

Circle, Lisa ;

Adkins, Hollie ;

Harbeck, Steven G. ;

Meyer, Eric D. ;

Bautista, Melissa A. ;

Martis, Prithy C. ;

Laramore, Melissa A. ;

Vinerean, Horatiu V. ;

Hall, Richard D. ;

Smith, Barry H. .

CELL TRANSPLANTATION, 2014, 23 (08) :929-944

[12] MSCs promote the development and improve the function of neonatal porcine islet grafts [J].

He, Sirong ;

Wang, Chengshi ;

Du, Xiaojiong ;

Chen, Younan ;

Zhao, Jiuming ;

Tian, Bole ;

Lu, Huimin ;

Zhang, Yi ;

Liu, Jingping ;

Yang, Guang ;

Li, Lan ;

Li, Hongxia ;

Cheng, Jingqiu ;

Lu, Yanrong .

FASEB JOURNAL, 2018, 32 (06) :3242-3253

[13] Culturing with modified EGM2 medium enhances porcine neonatal islet-like cell clusters resistance to apoptosis in islet xenotransplantation [J].

Ma, Xiaoqian ;

Yang, Cejun ;

Zhang, Juan ;

Wang, Jia ;

Li, Wei ;

Xu, Chang ;

Rong, Pengfei ;

Ye, Bin ;

Wu, Minghua ;

Jiang, Jianhui ;

Yi, Shounan ;

Wang, Wei .

XENOTRANSPLANTATION, 2018, 25 (01)

[14] Early barriers to neonatal porcine islet engraftment in a dual transplant model [J].

Samy, K. P. ;

Davis, R. P. ;

Gao, Q. ;

Martin, B. M. ;

Song, M. ;

Cano, J. ;

Farris, A. B. ;

McDonald, A. ;

Gall, E. K. ;

Dove, C. R. ;

Leopardi, F. V. ;

How, T. ;

Williams, K. D. ;

Devi, G. R. ;

Collins, B. H. ;

Kirk, A. D. .

AMERICAN JOURNAL OF TRANSPLANTATION, 2018, 18 (04) :998-1006

[15] Pseudotyping of porcine endogenous retrovirus by xenotropic murine leukemia virus in a pig islet xenotransplantation model [J].

Martina, Y ;

Kurian, S ;

Cherqui, S ;

Evanoff, G ;

Wilson, C ;

Salomon, DR .

AMERICAN JOURNAL OF TRANSPLANTATION, 2005, 5 (08) :1837-1847

[16] Islet-derived damage-associated molecular pattern molecule contributes to immune responses following microencapsulated neonatal porcine islet xenotransplantation in mice [J].

Itoh, Takeshi ;

Hata, Yuko ;

Nishinakamura, Hitomi ;

Kumano, Kenjiro ;

Takahashi, Hiroyuki ;

Kodama, Shohta .

XENOTRANSPLANTATION, 2016, 23 (05) :393-404

[17] In vitro characterization of neonatal, juvenile, and adult porcine islet oxygen demand, β-cell function, and transcriptomes [J].

Smith, Kate E. ;

Purvis, William G. ;

Davis, Melissa A. ;

Min, Catherine G. ;

Cooksey, Amanda M. ;

Weber, Craig S. ;

Jandova, Jana ;

Price, Nicholas D. ;

Molano, Diana S. ;

Stanton, James Brett ;

Kelly, Amy C. ;

Steyn, Leah V. ;

Lynch, Ronald M. ;

Limesand, Sean W. ;

Alexander, Michael ;

Lakey, Jonathan R. T. ;

Seeberger, Karen ;

Korbutt, Gregory S. ;

Mueller, Kate R. ;

Hering, Bernhard J. ;

McCarthy, Fiona M. ;

Papas, Klearchos K. .

XENOTRANSPLANTATION, 2018, 25 (06)

[18] Effects of anti-oxidizing vitamins on in vitro cultured porcine neonatal pancreatic islet cells [J].

Luca, G ;

Nastruzzi, C ;

Basta, G ;

Brozzetti, A ;

Saturni, A ;

Mughetti, D ;

Ricci, M ;

Rossi, C ;

Brunetti, P ;

Calafiore, R .

DIABETES NUTRITION & METABOLISM, 2000, 13 (06) :301-307

[19] The effect of hypoxia on free and encapsulated adult porcine islets-an in vitro study [J].

Muthyala, Sudhakar ;

Safley, Susan ;

Gordan, Kereen ;

Barber, Graham ;

Weber, Collin ;

Sambanis, Athanassios .

XENOTRANSPLANTATION, 2017, 24 (01)

[20] Formation of Re-Aggregated Neonatal Porcine Islet Clusters Improves In Vitro Function and Transplantation Outcome [J].

Honarpisheh, M. ;

Lei, Y. ;

Zhang, Y. ;

Pehl, M. ;

Kemter, E. ;

Kraetzl, M. ;

Lange, A. ;

Wolf, E. ;

Wolf-van Buerck, L. ;

Seissler, J. .

TRANSPLANT INTERNATIONAL, 2022, 35

← 1 2 3 4 →