Introducing cymantrene labels into scattering scanning near-field infrared microscopy

被引:4
作者
Kopf, Ilona [1 ]
N'Dongo, Harmel W. Peindy [1 ]
Ballout, Fouad [1 ]
Schatzschneider, Ulrich [1 ]
Bruendermann, Erik [1 ]
Havenith, Martina [1 ]
机构
[1] Ruhr Univ Bochum, Bochum, Germany
关键词
CARBONYL METALLO-IMMUNOASSAY; CARBONYLMETALLOIMMUNOASSAY CMIA; GOLD SURFACES; SPECTROSCOPY; RESOLUTION; CYTOTOXICITY; MONOLAYERS; SNIM;
D O I
10.1039/c2an16201f
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this paper we investigate metal-organic compounds as infrared (IR) active labels by scattering scanning near-field infrared microscopy (IR s-SNOM, often also abbreviated as s-SNIM) with a lateral resolution of 90 x 90 nm(2). Tailor-made IR spectroscopic probes based on cymantrene (CpMn(CO)(3) with Cp = eta(5)-C5H5) conjugated to a cysteine-modified pseudoneurotensin (pNT-Cys-OH) peptide were prepared by automated microwave-assisted solid phase peptide synthesis (SPPS) and characterized by HPLC, ESI-MS and IR. Well-defined patterned self-assembled monolayers on a gold surface were prepared by microcontact printing of 1-octadecanethiol (ODT) followed by additional incubation in ethanolic solution of the cymantrene-peptide derivative. The self-assembled monolayers have been evidenced by infrared reflection absorption spectroscopy (IRRAS) and AFM. CO laser source radiation was tuned (1944, 1900, 1798 and 1658 cm(-1)) for imaging contrast with good matching correlation between spectroscopic and topographic patterns at specific characteristic metal carbonyl and amide bands (1944 cm(-1) (lambda = 5.14 mu m) and 1658 cm(-1) (lambda = 6.03 mu m)). Cymantrene probes provide an attractive method to tag a unique spectroscopic feature on any bio(macro)molecule. Introducing such probes into super-resolution IR s-SNOM will enable molecular tracking and distribution studies even in complex biological systems.
引用
收藏
页码:4995 / 5001
页数:7
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