Effect of SsrA (tmRNA) tagging system on translational regulation in Streptomyces

被引：13

作者：

Braud, S ^{[1
]}

Lavire, C ^{[1
]}

Bellier, A ^{[1
]}

Mazodier, P ^{[1
]}

机构：

[1] Inst Pasteur, UP Genet Bacterienne & Differenciat, F-75724 Paris 15, France

来源：

ARCHIVES OF MICROBIOLOGY | 2006年 / 184卷 / 06期

关键词：

Streptomyces; differentiation; ssrA; tmRNA; rare codon; bldA; protease;

D O I：

10.1007/s00203-005-0051-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

ssrA genes encoding tmRNA with transfer and messenger RNA functions are ubiquitous in bacteria. In a process called trans-translation, tmRNA enters a stalled ribosome and allows release of the original mRNA, then tmRNA becomes the template for translation of a short tag that signals for proteolytic degradation. We provide here the first evidences that the tmRNA tagging system (ssrA and cohort smpB) is active in Streptomyces. Transcription of the genes was shown and construction of a genetic probe allowed detection of a tmRNA-tagged peptide. Obtention of ssrA and smpB mutants of Streptomyces lividans showed that the ssrA system is dispensable in Streptomyces. Morphologies of the mutants colonies were similar to the wild type, thus tmRNA-mediated tagging does not seem to have, under conditions used, a significant effect in the Streptomyces differentiation.

引用

页码：343 / 352

页数：10

共 48 条

[1] SsrA-mediated protein tagging in the presence of miscoding drugs and its physiological role in Escherichia coli [J].

Abo, T ;

Ueda, K ;

Sunohara, T ;

Ogawa, K ;

Aiba, H .

GENES TO CELLS, 2002, 7 (07) :629-638

[2] Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2) [J].

Bentley, SD ;

Chater, KF ;

Cerdeño-Tárraga, AM ;

Challis, GL ;

Thomson, NR ;

James, KD ;

Harris, DE ;

Quail, MA ;

Kieser, H ;

Harper, D ;

Bateman, A ;

Brown, S ;

Chandra, G ;

Chen, CW ;

Collins, M ;

Cronin, A ;

Fraser, A ;

Goble, A ;

Hidalgo, J ;

Hornsby, T ;

Howarth, S ;

Huang, CH ;

Kieser, T ;

Larke, L ;

Murphy, L ;

Oliver, K ;

O'Neil, S ;

Rabbinowitsch, E ;

Rajandream, MA ;

Rutherford, K ;

Rutter, S ;

Seeger, K ;

Saunders, D ;

Sharp, S ;

Squares, R ;

Squares, S ;

Taylor, K ;

Warren, T ;

Wietzorrek, A ;

Woodward, J ;

Barrell, BG ;

Parkhill, J ;

Hopwood, DA .

NATURE, 2002, 417 (6885) :141-147

[3] Antibiotic resistance gene cassettes derived from the Omega interposon for use in E-coli and Streptomyces [J].

BlondeletRouault, MH ;

Weiser, J ;

Lebrihi, A ;

Branny, P ;

Pernodet, JL .

GENE, 1997, 190 (02) :315-317

[4] Screening for stabilization of proteins with a trans-translation signature in Escherichia coli selects for inactivation of the ClpXP protease [J].

Bohn, C ;

Binet, E ;

Bouloc, P .

MOLECULAR GENETICS AND GENOMICS, 2002, 266 (05) :827-831

[5]

CHATER KF, 1993, ANNU REV MICROBIOL, V47, P685, DOI 10.1146/annurev.mi.47.100193.003345

[6] Taking a genetic scalpel to the Streptomyces colony [J].

Chater, KF .

MICROBIOLOGY-UK, 1998, 144 :1465-1478

[7] A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans [J].

Chaveroche, Marie-Kim ;

Ghigo, Jean-Marc ;

d'Enfert, Christophe .

NUCLEIC ACIDS RESEARCH, 2000, 28 (22) :E97

[8] Competition between SsrA tagging and translational termination at weak stop codons in Escherichia coli [J].

Collier, J ;

Binet, E ;

Bouloc, P .

MOLECULAR MICROBIOLOGY, 2002, 45 (03) :745-754

[9] One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].

Datsenko, KA ;

Wanner, BL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645

[10] Alteration of the synthesis of the Clp ATP-dependent protease affects morphological and physiological differentiation in Streptomyces [J].

de Crécy-Lagard, V ;

Servant-Moisson, P ;

Viala, J ;

Grandvalet, C ;

Mazodier, P .

MOLECULAR MICROBIOLOGY, 1999, 32 (03) :505-517

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