A luminum-phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

被引：70

作者：

Muehlmann, Luis Alexandre ^{[1
]}

Ma, Beatriz Chiyin ^{[1
]}

Figueiro Longo, Joao Paulo ^{[1
]}

Menezes, Maria de Fatima ^{[1
]}

Santos, Almeida ^{[1
]}

Azevedo, Ricardo Bentes ^{[1
]}

机构：

[1] Univ Brasilia, Inst Biol Sci, Dept Genet & Morphol, Brasilia, DF, Brazil

来源：

INTERNATIONAL JOURNAL OF NANOMEDICINE | 2014年 / 9卷

关键词：

third-generation photosensitizer; nanoparticles; cancer; photodynamic therapy; drug delivery systems; CELL-DEATH; DELIVERY-SYSTEM; IN-VITRO; ZINC PHTHALOCYANINE; NECROSIS; AGGREGATION; MECHANISMS; APOPTOSIS; GROWTH; ASSAY;

D O I：

10.2147/IJN.S57420

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Photodynamic therapy is generally considered to be safer than conventional anticancer therapies, and it is effective against different kinds of cancer. However, its clinical application has been significantly limited by the hydrophobicity of photosensitizers. In this work, a system composed of the hydrophobic photosensitizer aluminum-phthalocyanine chloride (AlPc) associated with water dispersible poly(methyl vinyl ether-co-maleic anhydride) nanoparticles is described. AlPc was associated with nanoparticles produced by a method of solvent displacement. This system was analyzed for its physicochemical characteristics, and for its photodynamic activity in vitro in cancerous (murine mammary carcinoma cell lineage 4T1, and human mammary adenocarcinoma cells MCF-7) and noncancerous (murine fibroblast cell lineage NIH/3T3, and human mammary epithelial cell lineage MCF-10A) cell lines. Cell viability and the elicited mechanisms of cell death were evaluated after the application of photodynamic therapy. This system showed improved photophysical and photochemical properties in aqueous media in comparison to the free photosensitizer, and it was effective against cancerous cells in vitro.

引用

页码：1199 / 1213

页数：15

共 51 条

[21]

Longo J. Figueiro, 2012, CHEMOTHERAPY, V1, P2, DOI DOI 10.1016/J.APSB.2012.07.004

[22]

LOTAN R, 1979, CANCER RES, V39, P1014

[23] Activatable Photosensitizers for Imaging and Therapy [J].

Lovell, Jonathan F. ;

Liu, Tracy W. B. ;

Chen, Juan ;

Zheng, Gang .

CHEMICAL REVIEWS, 2010, 110 (05) :2839-2857

[24] Initiation of apoptosis versus necrosis by photodynamic therapy with chloroaluminum phthalocyanine [J].

Luo, Y ;

Kessel, D .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1997, 66 (04) :479-483

[25] Basic principles of photodynamic therapy [J].

MacDonald, IJ ;

Dougherty, TJ .

JOURNAL OF PORPHYRINS AND PHTHALOCYANINES, 2001, 5 (02) :105-129

[26] Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review [J].

Maeda, H ;

Wu, J ;

Sawa, T ;

Matsumura, Y ;

Hori, K .

JOURNAL OF CONTROLLED RELEASE, 2000, 65 (1-2) :271-284

[27] Necrotic and apoptotic features of cell death in response to Foscan® photo sensitization of HT29 monolayer and multicell spheroids [J].

Marchal, S ;

Fadloun, A ;

Maugain, E ;

D'Hallewin, MA ;

Guillemin, F ;

Bezdetnaya, L .

BIOCHEMICAL PHARMACOLOGY, 2005, 69 (08) :1167-1176

[28] Delivery of the Photosensitizer Pc 4 in PEG-PCL Micelles for In Vitro PDT Studies [J].

Master, Alyssa M. ;

Rodriguez, Myriam E. ;

Kenney, Malcolm E. ;

Oleinick, Nancy L. ;

Sen Gupta, Anirban .

JOURNAL OF PHARMACEUTICAL SCIENCES, 2010, 99 (05) :2386-2398

[29] Photodynamic therapy for prostate cancer-a review of current status and future promise [J].

Moore, Caroline M. ;

Pendse, Doug ;

Emberton, Mark .

NATURE CLINICAL PRACTICE UROLOGY, 2009, 6 (01) :18-30

[30] RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS [J].

MOSMANN, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 65 (1-2) :55-63

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