N-acylhydrazone improves exercise intolerance in rats submitted to myocardial infarction by the recovery of calcium homeostasis in skeletal muscle

被引：14

作者：

da Silva, Jaqueline Soares ^{[1
]}

Pereira, Sharlene Lopes ^{[1
]}

Maia, Rodolfo do Couto ^{[1
]}

Landgraf, Sharon Schilling ^{[2
]}

Caruso-Neves, Celso ^{[2
]}

Kuemmerle, Arthur Eugen ^{[3
]}

Manssour Fraga, Carlos Alberto ^{[1
]}

Barreiro, Eliezer Jesus ^{[1
]}

Sudo, Roberto Takashi ^{[1
]}

Zapata-Sudo, Gisele ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Programa Desenvolvimento Farmacos, BR-21941590 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21941 Rio De Janeiro, Brazil

[3] Univ Fed Rural Rio de Janeiro, Inst Ciencias Exatas, Dept Quim, Seropedica, RJ, Brazil

来源：

LIFE SCIENCES | 2014年 / 94卷 / 01期

关键词：

Myocardial infarction; Exercise intolerance; LASSBio-294; SERCA2a expression; CHRONIC HEART-FAILURE; ANGIOTENSIN-II; EXPRESSION; INCREASES; THIENYLHYDRAZONE; POSTINFARCTION; LASSBIO-294; DYSFUNCTION; RELEASE; FIBERS;

D O I：

10.1016/j.lfs.2013.11.012

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aims: This work investigated the effects of 3,4-methylenedioxybenzoyl-2-thienylhydrazone (LASSBio-294) treatment on the contractile response of soleus (SOL) muscle from rats submitted to myocardial infarction (MI). Main methods: Following coronary artery ligation, LASSBio-294 (2 mg/kg, i.p.) or vehicle was administrated once daily for 4 weeks. Key findings: The run time to fatigue for sham rats was 17.9 +/- 2.6 min, and it was reduced to 3.3 +/- 0.8 min (P < 0.05) in MI rats. In MI rats treated with LASSBio-294, the time to fatigue was 15.1 +/- 3.6 min. During the contractile test, SOL muscles from sham rats showed a response of 7.12 +/- 0.54 N/cm(2) at 60 Hz, which was decreased to 5.45 +/- 0.49 N/cm(2) (P < 0.05) in MI rats. The contractility of SOL muscles from the MI-LASSBio-294 group was increased to 9.01 +/- 0.65 N/cm(2). At 16 mM caffeine, the contractility was reduced from 2.31 +/- 0.33 to 1.60 +/- 0.21 N/cm(2) (P < 0.05) in the MI group. In SOL muscles from MI-LASSBio-294 rats, the caffeine response was increased to 2.62 +/- 0.33 N/cm(2). Moreover, SERCA2a expression in SOL muscles was decreased by 0.31-fold (31%) in the MI group compared to the Sham group (P < 0.05). In the MI-LASSBio-294 group, it was increased by 1.53-fold (153%) compared to the MI group (P < 0.05). Meanwhile, the nuclear density in SOL muscles was increased in the MI group compared to the Sham group. Treatment with LASSBio-294 prevented this enhancement of cellular infiltrate. Significance: LASSBio-294 treatment prevented the development of muscular fatigue and improved exercise in-tolerance in rats submitted to MI. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：30 / 36

页数：7

共 38 条

[1] Cardiac output response to exercise in relation to metabolic demand in heart failure with preserved ejection fraction [J].