Carbon nanospheres mediated nuclear delivery of SMAR1 protein (DNA binding domain) controls breast tumor in mice model

被引：3
|
作者：
Bhagat, Prasad N. [1 ]
Jadhav, Sachin H. [1 ]
Chattopadhyay, Samit [2 ,3 ]
Paknikar, Kishore M. [1 ]
机构：
[1] Agharkar Res Inst, GG Agarkar Rd, Pune 411004, Maharashtra, India
[2] Natl Ctr Cell Sci, SP Pune Univ Campus,Ganeshkhind Rd, Pune 411007, Maharashtra, India
[3] Indian Inst Chem Biol, 4 Raja SC Mullick Rd, Kolkata 700032, India
来源：
NANOMEDICINE | 2018年 / 13卷 / 04期
关键词：
breast cancer; carbon nanospheres; DNA binding domain; PET; SMAR1; MESOPOROUS SILICA NANOPARTICLES; SOLID LIPID NANOPARTICLES; CANCER-THERAPY; IN-VIVO; P53; PEPTIDE; GROWTH; CELLS; THERAPEUTICS; METASTASIS;
D O I：
10.2217/nnm-2017-0298
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: To investigate anticancer activity of the DNA binding domain of SMAR1 (His 5) in vitro and in vivo. Materials & methods: His 5 was conjugated to hydrothermally synthesized carbon nanospheres (CNs). Anticancer activity of CNs-His 5 was evaluated in vitro and in vivo. Results: CNs-His 5 significantly reduced cyclin D1 levels in MDA-MB-231 cells. Tumor bearing Balb/c mice injected with CNs-His 5 showed approximately 62% tumor regression and significantly reduced (18)FDG uptake. Caspases assay and IHC staining confirmed tumor growth inhibition, which could be attributed to apoptotic, antiproliferative and antiangiogenic activities of His 5. Conclusion: DNA binding domain of the SMAR1 protein (His 5) has potent anticancer activity and its CNs mediated delivery could control breast tumor in mice model.
引用
收藏
页码：353 / 372
页数：20
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