Application of RP-TLC technique for the determination of dissociation constants of 1-substituted pyrrolidin-2-one derivatives

被引：3

作者：

Kulig, Katarzyna ^{[1
]}

Rybicka, Karolina ^{[1
]}

Malawska, Barbara ^{[1
]}

机构：

[1] Jagiellonian Univ, Coll Med, Dept Physicochem Drug Anal, Chair Pharmaceut Chem,Fac Pharm, PL-30688 Krakow, Poland

来源：

BIOMEDICAL CHROMATOGRAPHY | 2008年 / 22卷 / 11期

关键词：

dissociation constants; pK(a); RP-TLC; potentiometric titration; pyrrolidin-2-one derivatives;

D O I：

10.1002/bmc.1048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The procedures for measuring dissociation constants (pK(a)) of twenty 1-substituted pyrrolidin-2-one derivatives are described. The dissociation constants of the compounds tested were determined using potentiometric titration, reversed-phase thin-layer chromatography (RP-TLC) and calculated using Pallas and Marvin programs. It was found that the RP-TLC method of determination of pK(a) could be considered as a feasible alternative to potentiometric titration. The Marvin program is a better tool for preliminary estimation of dissociation constant than the Pallas one. Copyright (c) 2008 John Wiley & Sons, Ltd.

引用

页码：1225 / 1229

页数：5