The preparation of ethylenediamine-modified fluorescent carbon dots and their use in imaging of cells

被引：46
作者：
Dong, Wei [1 ]
Zhou, Siqi [2 ]
Dong, Yan [3 ]
Wang, Jingwen [1 ]
Ge, Xin [1 ]
Sui, Lili [1 ]
机构：
[1] Shenyang Med Coll, Dept Chem, Shenyang 110034, Peoples R China
[2] Fengtian Hosp, Shenyang Med Coll, ICU, Shenyang, Peoples R China
[3] Shenyang Pharmaceut Univ, Tradit Chinese Med Dept, Expt Ctr, Shenyang, Peoples R China
来源：
LUMINESCENCE | 2015年 / 30卷 / 06期
关键词：
carbon dots; ethylenediamine; cell imaging; cytotoxicity; CHEMILUMINESCENCE; NANOPARTICLES; NANODOTS;
D O I：
10.1002/bio.2834
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this work, fluorescent carbon dots (CDs) were synthesized using a hydrothermal method with glucose as the carbon source and were surface-modified with ethylenediamine. The properties of as-prepared CDs were analyzed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR), ultraviolet-visible light (UV/vis) absorption and fluorescent spectra. Furthermore, CDs conjugated with mouse anti-(human carcinoembryonic antigen) (CEA) monoclonal antibody were successful employed in the biolabeling and fluorescent imaging of human gastric carcinoma cells. In addition, the cytotoxicity of CDs was also tested using human gastric carcinoma cells. There was no apparent cytotoxicity on human gastric carcinoma cells. These results suggest the potential application of the as-prepared CDs in bioimaging and related fields. Copyright (C) 2015 John Wiley & Sons, Ltd.
引用
收藏
页码：867 / 871
页数：5
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