The preparation of ethylenediamine-modified fluorescent carbon dots and their use in imaging of cells

被引：49

作者：

Dong, Wei ^{[1
]}

Zhou, Siqi ^{[2
]}

Dong, Yan ^{[3
]}

Wang, Jingwen ^{[1
]}

Ge, Xin ^{[1
]}

Sui, Lili ^{[1
]}

机构：

[1] Shenyang Med Coll, Dept Chem, Shenyang 110034, Peoples R China

[2] Fengtian Hosp, Shenyang Med Coll, ICU, Shenyang, Peoples R China

[3] Shenyang Pharmaceut Univ, Tradit Chinese Med Dept, Expt Ctr, Shenyang, Peoples R China

来源：

LUMINESCENCE | 2015年 / 30卷 / 06期

关键词：

carbon dots; ethylenediamine; cell imaging; cytotoxicity; CHEMILUMINESCENCE; NANOPARTICLES; NANODOTS;

D O I：

10.1002/bio.2834

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, fluorescent carbon dots (CDs) were synthesized using a hydrothermal method with glucose as the carbon source and were surface-modified with ethylenediamine. The properties of as-prepared CDs were analyzed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR), ultraviolet-visible light (UV/vis) absorption and fluorescent spectra. Furthermore, CDs conjugated with mouse anti-(human carcinoembryonic antigen) (CEA) monoclonal antibody were successful employed in the biolabeling and fluorescent imaging of human gastric carcinoma cells. In addition, the cytotoxicity of CDs was also tested using human gastric carcinoma cells. There was no apparent cytotoxicity on human gastric carcinoma cells. These results suggest the potential application of the as-prepared CDs in bioimaging and related fields. Copyright (C) 2015 John Wiley & Sons, Ltd.

引用

页码：867 / 871

页数：5

共 24 条

[1] Isolation and characterization of fluorescent nanoparticles from pristine and oxidized electric arc-produced single-walled carbon nanotubes [J].

Bottini, M ;

Balasubramanian, C ;

Dawson, MI ;

Bergamaschi, A ;

Bellucci, S ;

Mustelin, T .

JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (02) :831-836

[2] Surface functionalized carbogenic quantum dots [J].

Bourlinos, Athanasios B. ;

Stassinopoulos, Andreas ;

Anglos, Demetrios ;

Zboril, Radek ;

Karakassides, Michael ;

Giannelis, Emmanuel P. .

SMALL, 2008, 4 (04) :455-458

[3] Carbon dots for multiphoton bioimaging [J].

Cao, Li ;

Wang, Xin ;

Meziani, Mohammed J. ;

Lu, Fushen ;

Wang, Haifang ;

Luo, Pengju G. ;

Lin, Yi ;

Harruff, Barbara A. ;

Veca, L. Monica ;

Murray, Davoy ;

Xie, Su-Yuan ;

Sun, Ya-Ping .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (37) :11318-+

[4]

Derfus AM, 2004, NANO LETT, V4, P11, DOI 10.1021/nl0347334

[5] Production of superoxide anion radicals as evidence for carbon nanodots acting as electron donors by the chemiluminescence method [J].

Dou, Xiangnan ;

Lin, Zhen ;

Chen, Hui ;

Zheng, Yongzan ;

Lu, Chao ;

Lin, Jin-Ming .

CHEMICAL COMMUNICATIONS, 2013, 49 (52) :5871-5873

[6] Characterization and application of single fluorescent nanodiamonds as cellular biomarkers [J].

Fu, Chi-Cheng ;

Lee, Hsu-Yang ;

Chen, Kowa ;

Lim, Tsong-Shin ;

Wu, Hsiao-Yun ;

Lin, Po-Keng ;

Wei, Pei-Kuen ;

Tsao, Pei-Hsi ;

Chang, Huan-Cheng ;

Fann, Wunshain .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (03) :727-732

[7] Polyethyleneimine modified fluorescent carbon dots and their application in cell labeling [J].

Han, Baofu ;

Wang, Wenxing ;

Wu, Hongyan ;

Fang, Fang ;

Wang, Naizhi ;

Zhang, Xiujuan ;

Xu, Shukun .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2012, 100 :209-214

[8] Involvement of autophagy in trypsinogen activation within the pancreatic acinar cells [J].

Hashimoto, Daisuke ;

Ohmuraya, Masaki ;

Hirota, Masahiko ;

Yamamoto, Akitsugu ;

Suyama, Koichi ;

Ida, Satoshi ;

Okumura, Yuushi ;

Takahashi, Etsuhisa ;

Kido, Hiroshi ;

Araki, Kimi ;

Baba, Hideo ;

Mizushima, Noboru ;

Yamamura, Ken-ichi .

JOURNAL OF CELL BIOLOGY, 2008, 181 (07) :1065-1072

[9] One-step synthesis of fluorescent carbon nanoparticles by laser irradiation [J].

Hu, Sheng-Liang ;

Niu, Kai-Yang ;

Sun, Jing ;

Yang, Jing ;

Zhao, Nai-Qin ;

Du, Xi-Wen .

JOURNAL OF MATERIALS CHEMISTRY, 2009, 19 (04) :484-488

[10] Gold nanoparticles induce oxidative damage in lung fibroblasts in vitro [J].

Li, Jasmine J. ;

Zou, Li ;

Hartono, Deny ;

Ong, Choon-Nam ;

Bay, Boon-Huat ;

Yung, Lin-Yue Lanry .

ADVANCED MATERIALS, 2008, 20 (01) :138-+

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