Comparison of two PCR and one ELISA techniques for the detection of small ruminant lentiviruses (SRLVs) in milk of sheep and goats

被引：17

作者：

Barquero, N. ^{[1
]}

Domenech, A. ^{[1
]}

Arjona, A. ^{[1
]}

Fernandez-Garayzabal, J. F. ^{[1
]}

Ruiz-Santa-Quiteria, J. A. ^{[1
]}

Gomez-Lucia, E. ^{[1
]}

机构：

[1] Univ Complutense Madrid, Dept Anim Hlth, Sch Vet Sci, E-28040 Madrid, Spain

来源：

RESEARCH IN VETERINARY SCIENCE | 2013年 / 94卷 / 03期

关键词：

SRLVs; MVV; CAEV; PCR; ELISA; Diagnosis; DIAGNOSTIC PERFORMANCE; INFECTED SHEEP; MAEDI-VISNA; SAMPLES; FLOCKS; BLOOD;

D O I：

10.1016/j.rvsc.2013.01.004

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The aim of the present study was to compare the efficiency of two PCR techniques for the diagnosis of small ruminant lentiviruses (SRLVs). Detection of the proviral genome by PCR, though sensitive, is difficult due to the heterogeneity of the SRLV genomes. One of the PCR techniques amplifies a fragment in the pol gene (pol-PCR) and the other PCR targets the LTR region of the proviral genome (LTR-PCR). Milk from 194 sheep and 163 goats from farms in the Central Spain was analyzed by both techniques and compared to results obtained by ELISA. When compared to the serologic assay, the agreement of both PCR techniques was very low (0.024 and 0.020 in sheep, and 0.124 and 0.114 in goats). In view of these results, it may be concluded that the efficacy of PCR for the diagnosis of SRLVs is low and a combination of PCR and ELISA should be used for diagnosis. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：817 / 819

页数：3

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